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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
TLDR
This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing. Expand
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
TLDR
This RNA-guided DNA recognition platform provides a simple approach for selectively perturbing gene expression on a genome-wide scale and can efficiently repress expression of targeted genes in Escherichia coli, with no detectable off-target effects. Expand
The new frontier of genome engineering with CRISPR-Cas9
TLDR
The power of the CRISPR-Cas9 technology to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders is illustrated. Expand
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
TLDR
It is shown that both binding and cleavage of DNA by Cas9–RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM) and that PAM interactions trigger Cas9 catalytic activity. Expand
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
TLDR
The results establish that the CRISPR system can be used as a modular and flexible DNA-binding platform for the recruitment of proteins to a target DNA sequence, revealing the potential of CRISpri as a general tool for the precise regulation of gene expression in eukaryotic cells. Expand
Structural Basis for Double-Stranded RNA Processing by Dicer
TLDR
The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA substrates into small fragments about 25 nucleotides in length and itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end. Expand
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
TLDR
To compare the architectures and domain organization of diverse Cas9 proteins, the atomic structures of Cas9 from Streptococcus pyogenes and Actinomyces naeslundii and AnaCas9 were determined by x-ray crystallography and three-dimensional reconstructions of apo-SpyCas9, SpyCas9:RNA, and SpyCas 9:RNA:DNA were obtained by negative-stain single-particle electron microscopy. Expand
RNA-guided genetic silencing systems in bacteria and archaea
TLDR
Understanding how small RNAs are used to find and destroy foreign nucleic acids will provide new insights into the diverse mechanisms of RNA-controlled genetic silencing systems. Expand
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
TLDR
It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes. Expand
Structural basis for CRISPR RNA-guided DNA recognition by Cascade
TLDR
The composition and low-resolution structure of Cascade is presented and it is shown how it recognizes double-stranded DNA targets in a sequence-specific manner and suggests that continuous invader DNA surveillance takes place without energy investment. Expand
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