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Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures.
The three-dimensional culture of MCF-10A mammary epithelial cells on a reconstituted basement membrane results in formation of polarized, growth-arrested acini-like spheroids that recapitulate… Expand
The Role of Apoptosis in Creating and Maintaining Luminal Space within Normal and Oncogene-Expressing Mammary Acini
- J. Debnath, K. Mills, N. L. Collins, M. Reginato, S. Muthuswamy, J. Brugge
- Biology, Medicine
- 4 October 2002
We have utilized in vitro three-dimensional epithelial cell cultures to analyze the role of apoptosis in the formation and maintenance of a hollow glandular architecture. Lumen formation is… Expand
Modelling glandular epithelial cancers in three-dimensional cultures
Little is known about how the genotypic and molecular abnormalities associated with epithelial cancers actually contribute to the histological phenotypes observed in tumours in vivo. 3D epithelial… Expand
Inhibition of mTOR by Rapamycin Abolishes Cognitive Deficits and Reduces Amyloid-β Levels in a Mouse Model of Alzheimer's Disease
Background Reduced TOR signaling has been shown to significantly increase lifespan in a variety of organisms , , , . It was recently demonstrated that long-term treatment with rapamycin,… Expand
Integrins and EGFR coordinately regulate the pro-apoptotic protein Bim to prevent anoikis
Epithelial cells must adhere to the extracellular matrix (ECM) for survival, as detachment from matrix triggers apoptosis or anoikis. Integrins are major mediators of adhesion between cells and ECM… Expand
Requirement for Tec kinases Rlk and Itk in T cell receptor signaling and immunity.
T cell receptor (TCR) signaling requires activation of Zap-70 and Src family tyrosine kinases, but requirements for other tyrosine kinases are less clear. Combined deletion in mice of two Tec… Expand
Autophagy at the crossroads of catabolism and anabolism
Autophagy is a conserved catabolic process that degrades cytoplasmic constituents and organelles in the lysosome. Starvation-induced protein degradation is a salient feature of autophagy but recent… Expand
Molecular definitions of autophagy and related processes
Over the past two decades, the molecular machinery that underlies autophagic responses has been characterized with ever increasing precision in multiple model organisms. Moreover, it has become clear… Expand
Induction of autophagy during extracellular matrix detachment promotes cell survival.
- Christopher K. H. Fung, R. Lock, Sizhen Gao, Eduardo Salas, J. Debnath
- Biology, Medicine
- Molecular biology of the cell
- 19 December 2007
Autophagy has been proposed to promote cell death during lumen formation in three-dimensional mammary epithelial acini because numerous autophagic vacuoles are observed in the dying central cells… Expand
FoxO3a Directs a Protective Autophagy Program in Hematopoietic Stem Cells
Blood production is ensured by rare, self-renewing haematopoietic stem cells (HSCs). How HSCs accommodate the diverse cellular stresses associated with their life-long activity remains elusive. Here… Expand