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Functionalized membrane supports for covalent protein microsequence analysis.
- J. Coull, D. Pappin, J. Mark, R. Aebersold, H. Köster
- Chemistry, Medicine
- Analytical biochemistry
- 1 April 1991
Methods were developed for high yield covalent attachment of peptides and proteins to isothiocyanate and arylamine-derivatized poly(vinylidene difluoride) membranes for solid-phase sequence analysis.… Expand
PNA solubility enhancers
- B. Gildea, Shelagh Casey, J. Macneill, H. Perry-O'Keefe, Ditte Sørensen, J. Coull
- 1 October 1998
Abstract Fmoc-protected hydrophilic amino acids were prepared and incorporated into peptide nucleic acid (PNA). N- and C-terminal modification of PNA did not affect its ability to hybridize to… Expand
A novel method for the introduction of an aliphatic primary amino group at the 5' terminus of synthetic oligonucleotides
Abstract Reaction of the 5′ hydroxyl group of a support bound synthetic oligonucleotide with the new reagent N-trifluoroacetyl-2-aminoethyl-(2-cyanoethyl)-N,N-diisopropylaminophosphor-amidite yields,… Expand
A rapid fluorescent staining procedure for proteins electroblotted onto PVDF membranes
Membranes as solid supports for peptide synthesis
Abstract A hydroxypropylacrylate coated polypropylene membrane was used as a solid support for the stepwise synthesis of peptides. This novel support can either be used for the preparative synthesis… Expand
Solid-phase sequence analysis of proteins electroblotted or spotted onto polyvinylidene difluoride membranes.
Electroblotted proteins noncovalently bound to polyvinylidene difluoride (PVDF) membranes are typically sequenced using adsorptive sequencer protocols (gas-phase or pulsed-liquid) that do not require… Expand
Biochemical evidence that a D-loop is part of a four-stranded PNA-DNA bundle. Nickel-mediated cleavage of duplex DNA by a Gly-Gly-His bis-PNA.
A peptide nucleic acid (PNA) with improved strand-displacement capability and a site-specific DNA cleavage function is a novel reagent for probing the structure of PNA-DNA complexes in solution. By… Expand
Introduction of 5'-terminal functional groups into synthetic oligonucleotides for selective immobilization.
Oligodeoxyribonucleotides terminating in a 5'-primary amine group are synthesized using solid-phase supported phosphoramidite chemistry. The 5'-terminal amine group in the deprotected oligomers is… Expand
Development of Membrane Supports for the Solid-phase Sequence Analysis of Proteins and Peptides
The solid phase method of protein sequence analysis (Laursen, 1971) requires the covalent attachment of a peptide or protein to a solid matrix that is stable to the reaction conditions of the Edman… Expand