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Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas corrugata
Serology will not be a useful method for routine diagnosis of P. corrugata unless common antigens can be identified, and a correlation between LPS groups and serological reaction was observed. Expand
Structural determination of a 5-acetamido-3,5,7, 9-tetradeoxy-7-(3-hydroxybutyramido)-L-glycero-L-manno-nonulos onic acid-containing homopolysaccharide isolated from Sinorhizobium fredii HH103.
The K-antigen polysacharide of S. fredii HH103 is a homopolysaccharide, which is composed of a disaccharide repeating unit and the sugar residues are attached via a glycosidic linkage to the OH group of the 3-hydroxybutyramido substituent. Expand
Precipitation of Metallic Cations by the Acidic Exopolysaccharides from Bradyrhizobium japonicum and Bradyrhizobium (Chamaecytisus) Strain BGA-1
The results suggest that the precipitation is based on the interaction between carboxylate groups from different polysaccharide chains and the partially hydrolyzed aquoions of Fe, Al, Th, and Sn. Expand
Cooperative ATP inhibition and fructose-1,6-biphosphate activation of rat liver pyruvate kinase.
The results obtained on FDP activation show a great cooperativity for FDP saturation with a Hill coefficient of h = 2.79, which may contribute to explain the regulation of rat liver pyruvate kinase accounting for the activity of this enzyme at high FDP concentrations modulated by small changes in ATP concentrations. Expand
Alcian blue fixation allows silver staining of the isolated polysaccharide component of bacterial lipopolysaccharides in polyacrylamide gels
Treatment with Alcian Blue may prove useful to detect hydrophilic components of lipopolysaccharide samples that could no longer be stained by the usual silver staining procedures. Expand
Time, the forgotten dimension of ligand binding teaching
  • J. Corzo
  • Chemistry, Medicine
  • Biochemistry and molecular biology education : a…
  • 1 November 2006
A brief discussion and some examples useful for teaching the importance of time in ligand binding by macromolecules in the context of a general biochemistry course are presented. Expand
Characterization of an aromatic amino acid aminotransferase from Rhizobium leguminosarum biovar trifolii.
The most abundant aromatic amino acid aminotransferase of Rhizobium leguminosarum biovar trifolii was partially purified and used aromatic keto acids and alpha-ketoglutaric and oxalacetic acids as amino-group acceptors. Expand
Effect of thorium on the growth and capsule morphology of Bradyrhizobium.
A specific defence mechanism based on metal precipitation by extracellular polymers is suggested, which is mainly in the amorphous fraction of Bradyrhizobium (Chamaecytisus) BGA-1. Expand
Aromatic amino acid aminotransferases in Rhizobium leguminosarum biovar Trifolii. Their role in indoleacetic acid synthesis
Five aromatic amino acid aminotransferases in this bacterium were detected which can be distinguished by electrophoretic mobility, ion-exchange chromatography and activity on histidine and none of them was active on nonaromatic amino acids other than histidine. Expand
Solution conformation and dynamics of an extracellular polysaccharide isolated from Bradyrhyzobium as deduced from 1H-NMR off resonance ROESY and 13C-NMR relaxation measurements.
The analysis of the data indicates the existence of important flexibility for the different linkages of the polysaccharide, and segmental motions as well as internal motions around the glycosidic linkages are the major sources of relaxation for this molecule at 299 K. Expand