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Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry.
Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an electron to multiplyExpand
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Parallel Reaction Monitoring for High Resolution and High Mass Accuracy Quantitative, Targeted Proteomics*
Selected reaction monitoring on a triple quadrupole mass spectrometer is currently experiencing a renaissance within the proteomics community for its, as yet, unparalleled ability to characterize andExpand
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Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry
We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-minExpand
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The utility of ETD mass spectrometry in proteomic analysis.
Mass spectrometry has played an integral role in the identification of proteins and their post-translational modifications (PTM). However, analysis of some PTMs, such as phosphorylation, sulfonation,Expand
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A neutral loss activation method for improved phosphopeptide sequence analysis by quadrupole ion trap mass spectrometry.
Recent advances in phosphopeptide enrichment prior to mass spectrometric analysis show genuine promise for characterization of phosphoproteomes. Tandem mass spectrometry of phosphopeptide ions, usingExpand
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Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease*
Because of its availability, ease of collection, and correlation with physiology and pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack of comparable dataExpand
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Performance Characteristics of Electron Transfer Dissociation Mass Spectrometry*S
We performed a large scale study of electron transfer dissociation (ETD) performance, as compared with ion trap collision-activated dissociation (CAD), for peptides ranging from ∼1000 to 5000 Da (n ∼Expand
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Decision tree–driven tandem mass spectrometry for shotgun proteomics
Mass spectrometry has become a key technology for modern large-scale protein sequencing. Tandem mass spectrometry, the process of peptide ion dissociation followed by mass-to-charge ratio (m/z)Expand
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A dynamic model of proteome changes reveals new roles for transcript alteration in yeast
The transcriptome and proteome change dynamically as cells respond to environmental stress; however, prior proteomic studies reported poor correlation between mRNA and protein, rendering theirExpand
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Value of using multiple proteases for large-scale mass spectrometry-based proteomics.
Large-scale protein sequencing methods rely on enzymatic digestion of complex protein mixtures to generate a collection of peptides for mass spectrometric analysis. Here we examine the use ofExpand
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