• Publications
  • Influence
The calpain system.
The calpain system originally comprised three molecules: two Ca2+-dependent proteases, mu-calpain and m-calpain, and a third polypeptide, calpastatin, whose only known function is to inhibit the twoExpand
  • 2,463
  • 249
The role of autolysis in activity of the Ca2+-dependent proteinases (mu-calpain and m-calpain).
A recent hypothesis suggests that proteolytic activity of the micromolar and millimolar Ca2+-requiring forms of the Ca2+-dependent proteinases (mu- and m-calpain, respectively) is regulated in vivoExpand
  • 155
  • 6
A BODIPY fluorescent microplate assay for measuring activity of calpains and other proteases.
The use of 4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-propionic acid (BODIPY-FL) labeled casein in autoquenching assays of proteolytic activity has been recently described, and weExpand
  • 95
  • 3
Site-directed mutagenesis of colicin E1 provides specific attachment sites for spin labels whose spectra are sensitive to local conformation.
Colicin E1 is an E. coli plasmid-encoded water-soluble protein that spontaneously inserts into lipid membranes to form a voltage-gated ion channel. We have employed a novel approach in whichExpand
  • 77
  • 2
Immunoaffinity purification of calpastatin and calpastatin constructs.
It has been difficult to purify calpastatin without using a step involving heating to 90-100 degrees C. Preparations of calpastatin obtained after heating often contain several polypeptides that haveExpand
  • 6
  • 1
Localization of the Ca2+‐dependent proteinases and their inhibitor in normal, fasted, and denervated rat skeletal muscle
Immunofluorescence and immunogold localization studies show that the two Ca2+‐dependent proteinases (μ‐calpain for the micromolar Ca2+‐requiring proteinase and m‐calpain for the millimolarExpand
  • 72
Localization of the Ca(2+)-dependent proteinases and their inhibitor in normal, fasted, and denervated rat skeletal muscle.
Immunofluorescence and immunogold localization studies show that the two Ca(2+)-dependent proteinases (mu-calpain for the micromolar Ca(2+)-requiring proteinase and m-calpain for the millimolarExpand
  • 41
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains.
Nine monoclonal antibodies (mAbs) specific for the 28-kDa subunit common to mu- and m-calpains have been assayed for their effects on mu- and m-calpains. All nine react with the COOH-terminal partExpand
  • 18
The calpain system in human placenta.
The calpain system is involved in a number of human pathologies ranging from the muscular dystrophies to Alzheimer's disease. It is important, therefore, to be able to obtain and to characterize bothExpand
  • 11
Immunoaffinity purification of the calpains.
A monoclonal antibody to the small subunit common to both mu- and m-calpains can be used in an immunoaffinity column to purify either mu- or m-calpain in a proteolytically active form. Extracts inExpand
  • 14