Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
- D. Klionsky, K. Abdelmohsen, S. Zughaier
- BiologyAutophagy
- 18 April 2012
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
Obesity Induces Expression of Uncoupling Protein-2 in Hepatocytes and Promotes Liver ATP Depletion*
- K. Chavin, Shiqi Yang, A. Diehl
- BiologyJournal of Biological Chemistry
- 26 February 1999
In situ hybridization and immunohistochemical analysis of ob/ob livers demonstrate that U CP2 mRNA and protein expression are increased in hepatocytes, which do not express UCP2 in lean mice, and hepatocytes adapt to obesity by up-regulating UCP1.
Assessing bioenergetic function in response to oxidative stress by metabolic profiling.
- B. Dranka, G. Benavides, V. Darley-Usmar
- BiologyFree Radical Biology & Medicine
- 1 November 2011
Alterations in liver ATP homeostasis in human nonalcoholic steatohepatitis: a pilot study.
- H. Cortez‐Pinto, J. Chatham, V. P. Chacko, C. Arnold, A. Rashid, A. Diehl
- Medicine, BiologyJAMA
- 3 November 1999
Recovery from hepatic ATP depletion becomes progressively less efficient as body mass increases in healthy controls and is severely impaired in patients with obesity-related nonalcoholic steatohepatitis.
Glucosamine improves cardiac function following trauma-hemorrhage by increased protein O-GlcNAcylation and attenuation of NF-{kappa}B signaling.
- Luyun Zou, Shaolong Yang, J. Chatham
- Biology, ChemistryAmerican Journal of Physiology. Heart and…
- 1 February 2009
Results demonstrate that the modulation of O-GlcNAc levels alters the response of cardiomyocytes to the activation of the NF-kappaB pathway, which may contribute to the glucosamine-mediated improvement in cardiac function following hemorrhagic shock.
Importance of the bioenergetic reserve capacity in response to cardiomyocyte stress induced by 4-hydroxynonenal.
- B. Hill, B. Dranka, Luyun Zou, J. Chatham, V. Darley-Usmar
- BiologyBiochemical Journal
- 15 November 2009
It is found that intact rat neonatal ventricular myocytes exhibit a substantial bioenergetic reserve capacity under basal conditions; however, on exposure to pathologically relevant concentrations of HNE, oxygen consumption was increased until this reserve capacity was depleted, suggesting that oxidized lipids could contribute to myocyte injury by decreasing the bioener energetic reserve capacity.
STIM1/Orai1-mediated SOCE: current perspectives and potential roles in cardiac function and pathology.
- Helen E Collins, Xiaoyuan Zhu-Mauldin, R. Marchase, J. Chatham
- BiologyAmerican Journal of Physiology. Heart and…
- 15 August 2013
There is now substantial evidence that STIM1-mediated SOCE plays a key role in mediating cardiomyocyte hypertrophy, both in vitro and in vivo, and there is growing support for the contribution of SOCE to Ca²⁺ overload associated with ischemia/reperfusion injury.
Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference
- P. Barker, B. Soher, S. Blackband, J. Chatham, V. Mathews, R. Bryan
- ChemistryNMR in Biomedicine
- 1993
Using cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated, and choline and creatine concentrations are in good agreement with conventional biochemical values.
Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc.
- C. Teo, Sampat Ingale, G. Boons
- BiologyNature Chemical Biology
- 1 May 2010
The monoclonal antibodies made it possible to delineate differentially modified proteins of liver in response to trauma-hemorrhage and resuscitation in a rat model and suggest that the antibodies, in addition to their O-GlcNAc dependence, also appear to have different but overlapping local peptide determinants.
Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein O-GlcNAc and increased mitochondrial Bcl-2.
- V. Champattanachai, R. Marchase, J. Chatham
- BiologyAmerican Journal of Physiology - Cell Physiology
- 1 June 2008
The results demonstrate that the protective effects of glucosamine are mediated via increased formation of O-GlcNAc and suggest that this is due, in part, to enhanced mitochondrial Bcl-2 translocation.
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