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Isolation of an efficient actin promoter for use in rice transformation.
Deletion analysis of the Act1 5' intron suggests that the intron-mediated stimulation of GUS expression is associated, in part, with an in vivo requirement for efficient intron splicing. Expand
Requirement of a corepressor for Dr1-mediated repression of transcription.
DRAP1 was found to be expressed in all tissues analyzed with higher levels in tissues with a low mitotic index and a model for DRAP1-dependent, Dr1-mediated repression of transcription is proposed. Expand
Translational inhibition mediated by a short upstream open reading frame in the human cytomegalovirus gpUL4 (gp48) transcript
It is demonstrated that the coding information of the upstream open reading frame initiated by AUG2 (uORF2) is critical for the inhibitory signal, and a model of inhibition by the gp48 uORf2 signal is proposed. Expand
Coding sequence-dependent ribosomal arrest at termination of translation
Data support the hypothesis that the inhibition of downstream translation results from uORF2 peptide-dependent ribosomal arrest at termination and suggest that translation termination may be a regulatory step in expression of some eukaryotic genes. Expand
Translational inhibition by a human cytomegalovirus upstream open reading frame despite inefficient utilization of its AUG codon
The second of three short upstream open reading frames (uORF2) in the transcript leader of the human cytomegalovirus gp48 (gpUL4) virion glycoprotein gene inhibits downstream translation approximately 10-fold, and a model is proposed to explain this unprecedented example of a paradoxically strong inhibitory effect of an upstream ORF despite inefficient utilization of its initiation codon. Expand
Inhibition of nascent-peptide release at translation termination
Support is provided for a model in which the nascent uORF2 peptide blocks translation termination prior to hydrolysis of the peptidyl-tRNA bond, which results in ribosomal stalling on the transcript leader which in turn impedes the access of ribosomes to the downstream cistron. Expand
The chloroplast-located homolog of bacterial DNA recombinase.
The cDNA for the chloroplast-located homolog of bacterial RecA protein, designated recA-AT, was placed in a plasmid appropriate for in vitro transcription and translation, and purified to close to homogeneity using methods developed for E. coli RecA isolation. Expand
Ribosomal release without peptidyl tRNA hydrolysis at translation termination in a eukaryotic system.
The results suggest that a eukaryotic cellular mechanism exists for removing stalled ribosomes from mRNAs in the absence of peptidyl tRNA hydrolysis. Expand
Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene.
- Y. Wang, W. Zhang, J. Cao, D. Mcelroy, R. Wu
- Biology, Medicine
- Molecular and cellular biology
- 1 August 1992
A ubiquitous rice protein is identified which specifically recognizes this poly(dA-dT) element in the constitutively active Act1 promoter, and a CCCAA pentamer repeat-containing region was found to be a negative regulator of the Act 1 promoter in transformed rice protoplasts. Expand
Mutational analysis of the translational signal in the human cytomegalovirus gpUL4 (gp48) transcript leader by retroviral infection.
It is demonstrated that CMV gp48 uORF2 is a potent translational inhibitory element, independent of the cell type or expression system used, and on a transcript containing an additional kilobase of complex leader sequences. Expand