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Microbial conversion of steroid compounds: recent developments
Steroid compounds can be ranked among the most widely marketed products from the pharmaceutical industry. Highly specific reactions are required to produce functionalized compounds with therapeuticExpand
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Hydrophobic interaction chromatography of proteins.
In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present theExpand
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Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications.
Interest in producing large quantities of supercoiled plasmid DNA has recently increased as a result of the rapid evolution of gene therapy and DNA vaccines. Owing to the commercial interest in theseExpand
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Horseradish peroxidase: a valuable tool in biotechnology.
Peroxidases have conquered a prominent position in biotechnology and associated research areas (enzymology, biochemistry, medicine, genetics, physiology, histo- and cytochemistry). They are one ofExpand
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Cutinase: from molecular level to bioprocess development.
This review analyzes the role of cutinases in nature and their potential biotechnological applications. The cloning and expression of a fungal cutinase, Fusarium solani f. pisi, in Escherichia coliExpand
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Solvent tolerance in bacteria: role of efflux pumps and cross-resistance with antibiotics.
Microorganisms have mechanisms that enable them to tolerate lethal concentrations of toxic compounds. This feature has been exploited in a wide range of bioprocesses that range from bioremediationExpand
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Canine tumors: a spontaneous animal model of human carcinogenesis.
The enormous biologic complexity of human cancer has stimulated the development of more appropriate experimental models that could resemble in a natural and spontaneous manner the physiopathologicExpand
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Hematopoietic stem cells: from the bone to the bioreactor.
The ex vivo expansion of human hematopoietic stem cells is a rapidly developing area with a broad range of biomedical applications. The mechanisms of renewal, differentiation and plasticity of stemExpand
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Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks.
Gene therapy is a promising process for the prevention, treatment and cure of diseases such as cancer, acquired immunodeficiency syndrome (AIDS) and cystic fibrosis. One of the methods used toExpand
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Kinetic studies of the urease-catalyzed hydrolysis of urea in a buffer-free system
  • Y. Qin, J. Cabral
  • Chemistry, Medicine
  • Applied biochemistry and biotechnology
  • 1 December 1994
The kinetics of urea hydrolysis catalyzed by urease, mainly in the absence of buffers by use of the self-buffer effect of the products, was investigated. The effect of pH, temperature, andExpand
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