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Aflatoxin B1 transport in rat blood plasma. Binding to albumin in vivo and in vitro and spectrofluorimetric studies into the nature of the interaction.
Binding of [3H]aflatoxin B1 to rat plasma was investigated in vivo and in vitro. Column chromatographic and polyacrylamide gel electrophoretic analyses clearly demonstrated that aflatoxin B1 boundExpand
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Malic enzyme activity and related Biochemical aspects during ripening of γ-irradiated mango fruit
Abstract The increase in malic enzyme ( l -malate: NADP+ oxidoreductase (oxalacetate-decarboxylating) EC activity, usually observed during the r
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Substrate and phenobarbital inducible aflatoxin-4-hydroxylation and aflatoxin metabolism by rat liver microsomes.
Abstract A quantitative method based on the determination of the fluorescence of B 1 , B 2 , M 1 , M 2 , G 1 and a toxin called “GM 1 ” (most probably a 4-hydroxylated derivative of aflatoxin G 1 )Expand
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-cyclopiazonate oxidocyclase from Penicillium cyclopium. I. Assay methods, isolation and purification.
Abstract 1. 1. Spectrophotometric and colorimetric assay methods for the dehydrogenation of β-cyclopiazonic acid and the conversion of β- into α-cyclopiazonic acid, both catalysed by β-cyclopiazonateExpand
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Aflatoxin B1 alters protein phosphorylation in rat livers.
A study was conducted on the effect of aflatoxin B1 on protein phosphorylation in rat livers by incubation of soluble and insoluble cell fractions with [gamma 32P] ATP. SDS-polyacrylamide gelExpand
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Purification of an acidic deoxyribonuclease II from the primate spleen
Abstract 1. 1. Acid DNase from monkey and baboon spleens was isolated and purified by extraction at pH 3–4, (NH42SO4 fractionation, heating at 50° C for I hour, SP-C50 chromatography and SephadexExpand
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The effect of aflatoxin B 1 , aflatoxin B 2 and sterigmatocystin on nuclear deoxyribonucleases from rat and mouse livers.
There have been a number of in vitro and in vivo studies on the effects of various potent carcinogens such as the butter-yellow-type carcinogens, urethane, γ-butyrolactone and diepoxybutane on theExpand
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Characterization of the aflatoxin B1-binding site of rat albumin.
A fluorescence-enhancement method was used to investigate the non-covalent interaction between aflatoxin B1 and rat albumin. Solvent-induced shifts in the emission spectrum of aflatoxin B1 providedExpand
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The effects of aflatoxin B 1 and aminoazodye carcinogens on rat and mouse liver nuclear acid deoxyribonuclease.
Abstract The influence of a prolonged low level of administration to rats and mice of the potent hepatocarcinogen, B 1 (aflatoxin B 1 ) on liver nuclear acid DNAase was investigated. For comparison,Expand
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An aminopeptidase from Agave americana variegata--I: Isolation, purification and physical and chemical characterization.
Abstract 1. 1. An exopeptidase specific for the aminoterminus of peptides has been isolated and purified from Aciare Americana variegata by means of extraction, ammoniumsulphate fractionation,Expand
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