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Deep sequencing analysis of viruses infecting grapevines: Virome of a vineyard.
TLDR
It was found that Grapevine leafroll-associated virus 3 (GLRaV-3) is the most prevalent species, constituting 59% of the total reads, followed by Grapevine rupestris stem pitting- associated virus and Grapevine virus A.
Grapevine leafroll-associated virus 3
TLDR
The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs, and the application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction.
Modified cetyltrimethylammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction.
TLDR
The major modifications made to the protocol described here were a reduction of sample treatment steps and an increase in beta-mercaptoethanol concentration resulting in a robust, rapid and reproducible plant RNA extraction protocol that can be used for a broad range of plant species and tissue types.
Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase
TLDR
The manA gene was shown to be a superior selectable markers gene for improving transformation efficiencies when compared to antibiotic or herbicide selectable marker genes.
Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera.
TLDR
The aim of this work was to develop a VIGS vector based on the Grapevine virus A (GVA), which is a member of the genus Vitivirus, family Flexiviridae, which may constitute an important tool for improving functional genomics in V. vinifera.
Three genetic grapevine leafroll-associated virus 3 variants identified from South African vineyards show high variability in their 5′UTR
TLDR
Three genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) were identified in vineyards of the Western Cape, South Africa and the presence of two or three additional variant groups elsewhere in the world was supported.
Genetic variability within the coat protein gene of Grapevine fanleaf virus isolates from South Africa and the evaluation of RT-PCR, DAS-ELISA and ImmunoStrips as virus diagnostic assays.
TLDR
This is the first report on sequence analysis of full-length 2CCP gene cDNA clones of GFLV isolates from South Africa, finding rapid-direct-one-tube-RT-PCR to be the most reliable technique for detection.
Complete nucleotide sequence of a South African isolate of grapevine leafroll-associated virus 3 reveals a 5′UTR of 737 nucleotides
TLDR
The sequence of GLRaV-3, isolate GP18, has a 50UTR of 737 nt and the 30-terminus of GP18 was found to be similar to that of the NY-1 isolate, and the efficacy of RLM-RACE to determine the 50-termini of multiple ssRNA viruses from total RNA in a single reaction was investigated.
Genetic and physiological differences of European beech provenances (F. sylvatica L.) exposed to drought stress
TLDR
Significant changes in fine root δ 13 C and C/N ratio as well as the intrinsic Water Use Efficiency (iWUE) demonstrate a better adaptability to future environmental conditions of beech ecotypes genetically related to the Greek beech population.
Grapevine leafroll disease and associated viruses: a unique pathosystem.
TLDR
A systems biology approach using contemporary technologies in molecular biology, -omics, and cell biology aids in exploring the comparative molecular biology of GLRaVs and deciphering the complex network of host-virus-vector interactions to bridge the gap between genomics and phenomics of leafroll disease.
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