• Publications
  • Influence
Grapevine leafroll-associated virus 3
Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due toExpand
  • 122
  • 14
  • PDF
Deep sequencing analysis of viruses infecting grapevines: Virome of a vineyard.
Double stranded RNA, isolated from 44 pooled randomly selected vines from a diseased South African vineyard, has been used in a deep sequencing analysis to build a census of the viral population. TheExpand
  • 210
  • 13
Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera.
Virus-induced gene silencing (VIGS) is an attractive approach for studying gene function. Although the number of virus vectors available for use in VIGS experiments has increased in recent years,Expand
  • 64
  • 8
Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase
Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation systemExpand
  • 89
  • 8
Genetic variability within the coat protein gene of Grapevine fanleaf virus isolates from South Africa and the evaluation of RT-PCR, DAS-ELISA and ImmunoStrips as virus diagnostic assays.
Grapevine fanleaf virus (GFLV) is responsible for severe fanleaf degeneration in grapevines of all major wine producing regions of the world, including South Africa. In order to successfully controlExpand
  • 38
  • 8
Modified cetyltrimethylammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction.
A wide range of plant RNA extraction methods are available; however, many of these are limited in their application for a diverse range of plant species. With special emphasis on robustness andExpand
  • 63
  • 7
Three genetic grapevine leafroll-associated virus 3 variants identified from South African vineyards show high variability in their 5′UTR
Three genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) were identified in vineyards of the Western Cape, South Africa. The GLRaV-3 variants were identified by single-strandExpand
  • 41
  • 7
  • PDF
Complete nucleotide sequence of a South African isolate of grapevine leafroll-associated virus 3 reveals a 5′UTR of 737 nucleotides
Grapevine leafroll-associated virus 3 (GLRaV-3) is the type species of the genus Ampelovirus (family Closteroviridae) [1]. It is an economically important virus that is known to only infect VitisExpand
  • 50
  • 6
Complete nucleotide sequence of a new strain of grapevine leafroll-associated virus 3 in South Africa
The complete genome sequences of two newly identified genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) are described. Two isolates, GH11 (18671 nt) and GH30 (18576 nt) wereExpand
  • 30
  • 4
Grapevine leafroll disease and associated viruses: a unique pathosystem.
Grapevine leafroll is the most complex and intriguing viral disease of grapevine (Vitis spp.). Several monopartite closteroviruses (family Closteroviridae) from grapevines have been molecularlyExpand
  • 48
  • 4