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Stevia rebaudiana: Its agricultural, biological, and chemical properties
The purpose of this review is to summarize the existing agricultural, chemical and biochemical literature to provide a baseline for new research on stevia sweeteners.
Steviol glycoside biosynthesis.
Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana.
- A. Richman, A. Swanson, J. Brandle
- BiologyThe Plant journal : for cell and molecular…
- 11 November 2004
It is determined that steviol UGTs behave in a regioselective manner and propose a modified pathway for the synthesis of rebaudioside A from steviola, and an in vitro glucosyltransferase activity enzyme assay was conducted.
Hydrophobin Fusions for High-Level Transient Protein Expression and Purification in Nicotiana benthamiana1[W][OA]
- J. Joensuu, A. Conley, M. Lienemann, J. Brandle, M. Linder, R. Menassa
- BiologyPlant Physiology
- 11 December 2009
HFBI fusions increased the expression levels of plant-made recombinant proteins while also providing a simple means for their subsequent purification, and enhances the value of transient plant-based expression systems.
Heritability for yield, leaf:stem ratio and stevioside content estimated from a landrace cultivar of Stevia rebaudiana
Results show that improvement of stevia through selection is possible and that it has potential for production in southwestern Ontario, and response to selection, low-calorie sweetener.
Optimization of elastin‐like polypeptide fusions for expression and purification of recombinant proteins in plants
- A. Conley, J. Joensuu, A. Jevnikar, R. Menassa, J. Brandle
- Biology, EngineeringBiotechnology and bioengineering
- 15 June 2009
To determine the optimal ELP size for the accumulation of recombinant proteins and their subsequent purification, various ELP tags were fused to green fluorescent protein, interleukin‐10, erythropoietin and a single chain antibody fragment and then transiently expressed in tobacco leaves.
pORE: a modular binary vector series suited for both monocot and dicot plant transformation
A series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotylerpine plants is presented, minimizing all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently.
Induction of protein body formation in plant leaves by elastin-like polypeptide fusions
This study demonstrates that plant seed-specific factors are not required for the formation of protein bodies in vegetative plant tissues, suggesting that the endoplasmic reticulum possesses an intrinsic ability to form protein body-like accretions in eukaryotic cells when overexpressing particular proteins.
Genetic diversity in North American ginseng (Panax quinquefolius L.) grown in Ontario detected by RAPD analysis.
Lower levels of genetic diversity were detected among 3-year-old ginseng plants selected on the basis of greater plant height than among the plants randomly selected from the same subpopulation or over the whole population, suggesting that genetic factors at least partly contribute to morphological variation within the ginsENG population and that visual selection can be effective in identifying the genetic differences.