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Evidence for the presence of β-subunit of hexosaminidase in a case of Sandhoff disease using a blotting technique
- S. Gautron, L. Poenaru, J. Boue, H. Puissant, J. Lisman, J. Dreyfus
- Biology, Medicine
- Human Genetics
A technique involving successively electrophoresis of denatured proteins, transfer (blotting) onto nitrocellulose, and labelling by appropriate antibodies raised against the dissociated forms of hexosaminidases A and B allows the detection of α and β subunits in crude extracts of normal tissues. Expand