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The nature of Staphylococcus aureus MurA and MurZ and approaches for detection of peptidoglycan biosynthesis inhibitors
TLDR
Strains generated in this study were validated as potential tools for identifying novel anti‐staphylococcal agents targeting peptidoglycan biosynthesis using known inhibitors of the pathway. Expand
Increased mutability of Pseudomonas aeruginosa in biofilms.
TLDR
Down-regulation of antioxidant enzymes in P. aeruginosa biofilms may enhance the rate of mutagenic events due to the accumulation of DNA damage and provide a further source of antibiotic-resistant mutants in the CF lung. Expand
Functional and Biochemical Analysis of Chlamydia trachomatis MurC, an Enzyme Displaying UDP-N-Acetylmuramate:Amino Acid Ligase Activity
TLDR
It is demonstrated that the MurC domain of the Chlamydia trachomatis bifunctional protein is functionally expressed in Escherichia coli, since it complements a conditional lethal E. coli mutant possessing a temperature-sensitive lesion in MurC. Expand
The silver cation (Ag+): antistaphylococcal activity, mode of action and resistance studies.
TLDR
The rapid and extensive loss of membrane integrity observed upon challenge with Ag(+) suggests that the antibacterial activity results directly from damage to the bacterial membrane, suggesting that silver compounds remain a viable option for the prevention and treatment of topical staphylococcal infections. Expand
Biochemical characterisation of the chlamydial MurF ligase, and possible sequence of the chlamydial peptidoglycan pentapeptide stem
TLDR
The murF gene from Chlamydia trachomatis was shown to be capable of complementing a conditional Escherichia coli mutant impaired in UDP-MurNAc-tripeptide:d-Ala-d- ala ligase activity, which is expected for the chlamydial pentapeptide stem. Expand
Macrocyclic inhibitors of the bacterial cell wall biosynthesis enzyme MurD.
TLDR
Computer-based molecular design has been used to produce a series of new macrocyclic systems targeted against the bacterial cell wall biosynthetic enzyme MurD, which were found to show good inhibition when assayed against the MurD enzyme. Expand
Discovery of new inhibitors of the bacterial peptidoglycan biosynthesis enzymes MurD and MurF by structure-based virtual screening.
TLDR
This work has used the molecular docking programme eHiTS for the virtual screening of 1990 compounds from the National Cancer Institute 'Diversity Set' on MurD and MurF and provided four novel MurD inhibitors and one novel MurF inhibitor. Expand
Discovery of new inhibitors of D-alanine:D-alanine ligase by structure-based virtual screening.
TLDR
Three compounds were identified that inhibit the enzyme with K(i) values in micromolar range that have promising antibacterial activities against Gram-positive and Gram-negative bacteria. Expand
Assessment of a microplate method for determining the post-antibiotic effect in Staphylococcus aureus and Escherichia coli.
TLDR
The spectrophotometric microplate method for determining PAEs may be a suitable alternative to the classical method for those antibiotics that do not induce bacterial cell lysis. Expand
Functional and Biochemical Analysis of the Chlamydia trachomatis Ligase MurE
TLDR
It is demonstrated that the murE gene from Chlamydia trachomatis is capable of complementing a conditional Escherichia coli mutant impaired in UDP-MurNAc-L-Ala-D-Glu:meso-A(2)pm ligase activity. Expand
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