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Possible failure of NADP-glutamate dehydrogenase to participate directly in nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae.
Abstract The extent of repression exerted by various nitrogen-containing compounds was measured in wild type and NADP-GDH defective strains of Saccharomyces. We found that in strains carrying the… Expand
Quantitative analysis of two-dimensional electrophoretograms.
- J. Bossinger, M. Miller, K. P. Vo, E. Geiduschek, N. Xuong
- Biology, Medicine
- The Journal of biological chemistry
- 25 August 1979
A method for quantitative analysis of complex film density distributions in autoradiograms is described. The method is intended particularly for measuring the distribution of radioactivity among the… Expand
Molecular events associated with induction of arginase in Saccharomyces cerevisiae.
Arginase, the enzyme responsible for arginine degradation in Saccharomyces cerevisiae, is an inducible protein whose inhibition of ornithine carbamoyl-transferase has been studied extensively. Mutant… Expand
Selective inhibition of protein synthesis initiation in Saccharomyces cerevisiae by low concentrations of cycloheximide.
We have previously determined the amounts of time required to complete various macromolecular synthetic processes needed for induction of allophanate hydrolase in Saccharomyces cerevisiae. This… Expand
Nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae.
Saccharomyces cerevisiae can utilize allantoin as a sole nitrogen source by degrading it to ammonia, "CO(2)," and glyoxylate. We have previously shown that synthesis of the allantoin degradative… Expand
Sequence of molecular events involved in induction of allophanate hydrolase.
Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic… Expand
Tubulin and actin synthesis during brain development.
Chick brain proteins from 5- through 13-day embryos were labeled with l-[35S]methionine for 30 min in vitro and analyzed by two-dimensional gel electrophoresis. Autoradiographs of the gels were… Expand
Execution times of macromolecular synthetic processes involved in the induction of allophanate hydrolase at 15 degrees C.
We have observed that transcription, involved in production of allophanate hydrolase, is completed 2.5 min after the addition of inducer at 15 degrees C. The rna1 gene product must be functional up… Expand
The Development and Application of Expert Systems: A National Survey
The Annotator's Assistant: an expert system for direct submission of genetic sequence data
- J. Bossinger
- Computer Science, Medicine
- Comput. Appl. Biosci.
- 1 March 1988
The Annotator's Assistant is an expert system that runs on an IBM PC and helps the molecular biologist, who may have little knowledge of the structure or content of a GenBank entry, to construct a complete and valid sequence submission file. Expand