• Publications
  • Influence
DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency
Synthetic scaffolds that permit spatial and temporal organization of enzymes in living cells are a promising post-translational strategy for controlling the flow of information in both metabolic andExpand
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Ligand binding and allostery can emerge simultaneously
A heterotropic allosteric effect involves an effector molecule that is distinct from the substrate or ligand of the protein. How heterotropic allostery originates is an unanswered question. We haveExpand
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Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them.
Recombinant protein expression in Escherichia coli represents a cornerstone of the biotechnology enterprise. While cytoplasmic expression in this host has received the most attention, achievingExpand
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Screening and modular design for metabolic pathway optimization.
TLDR
This review focuses on two practices that increase the likelihood of finding a more advantageous pathway solution: implementing a screen to identify high producers and utilizing modular pathway design to streamline engineering efforts. Expand
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Universal genetic assay for engineering extracellular protein expression.
A variety of strategies now exist for the extracellular expression of recombinant proteins using laboratory strains of Escherichia coli . However, secreted proteins often accumulate in the cultureExpand
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Directed Evolution of Mycobacterium tuberculosis β-Lactamase Reveals Gatekeeper Residue That Regulates Antibiotic Resistance and Catalytic Efficiency
Directed evolution can be a powerful tool for revealing the mutational pathways that lead to more resistant bacterial strains. In this study, we focused on the bacterium Mycobacterium tuberculosis,Expand
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Engineered microbial biofuel production and recovery under supercritical carbon dioxide
Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. To solve these problems, we propose a high-pressure fermentation strategy,Expand
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Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells.
Heterologous expression of many proteins in bacteria, yeasts, and plants is often limited by low titers of functional protein. To address this problem, we have created a two-tiered directed evolutionExpand
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An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli.
The extracellular expression of recombinant proteins using laboratory strains of Escherichia coli is now routinely achieved using naturally secreted substrates, such as YebF or the osmoticallyExpand
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The ribosomal exit tunnel as a target for optimizing protein expression in Escherichia coli
The folding of many cellular proteins occurs co‐translationally immediately outside the ribosome exit tunnel, where ribosomal proteins and other associated factors coordinate the synthesis andExpand
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