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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localizedExpand
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Signals for sorting of transmembrane proteins to endosomes and lysosomes.
Sorting of transmembrane proteins to endosomes and lysosomes is mediated by signals present within the cytosolic domains of the proteins. Most signals consist of short, linear sequences of amino acidExpand
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Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
The cation-independent mannose 6-phosphate receptor (CI-MPR) mediates sorting of lysosomal hydrolase precursors from the TGN to endosomes. After releasing the hydrolase precursors into the endosomalExpand
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The Mechanisms of Vesicle Budding and Fusion
Genetic and biochemical analyses of the secretory pathway have produced a detailed picture of the molecular mechanisms involved in selective cargo transport between organelles. This transport occursExpand
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Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: Evidence for membrane cycling from Golgi to ER
Abstract In cells treated with brefeldin A (BFA), movement of newly synthesized membrane proteins from the endoplasmic reticulum (ER) to the Golgi apparatus was blocked. Surprisingly, theExpand
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Retrograde transport from endosomes to the trans-Golgi network
A subset of intracellular transmembrane proteins such as acid-hydrolase receptors, processing peptidases and SNAREs, as well as extracellular protein toxins such as Shiga toxin and ricin, undergoesExpand
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Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7
The retromer complex mediates retrograde transport of transmembrane cargo from endosomes to the trans-Golgi network (TGN). Mammalian retromer is composed of a sorting nexin (SNX) dimer that binds toExpand
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GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.
Formation of intracellular transport intermediates and selection of cargo molecules are mediated by protein coats associated with the cytosolic face of membranes. Here, we describe a novel family ofExpand
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Interaction of tyrosine-based sorting signals with clathrin-associated proteins.
Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system wasExpand
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Sorting of lysosomal proteins.
Lysosomes are composed of soluble and transmembrane proteins that are targeted to lysosomes in a signal-dependent manner. The majority of soluble acid hydrolases are modified with mannose 6-phosphateExpand
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