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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
Signals for sorting of transmembrane proteins to endosomes and lysosomes.
This work has shown that peptide motifs serve as a signal for sorting at various stages of the endosomal-lysosomal system and several proteins, including clathrin, AP-2, and Dab2, have been proposed to function as recognition proteins for NPXY signals.
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
Observations indicate that retromer prevents the delivery of the CI-MPR to lysosomes, probably by sequestration into endosome-derived tubules from where the receptor returns to the TGN.
Retrograde transport from endosomes to the trans-Golgi network
A central role for a 'tubular endosomal network' in sorting to recycling pathways that lead not only to the trans-Golgi network but also to different plasma-membrane domains and to specialized storage vesicles is proposed.
Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7
It is demonstrated that Rab5 and Rab7 act in concert to regulate retromer recruitment to endosomes.
GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.
Observations suggest that GGAs are components of ARF-regulated coats that mediate protein trafficking at the TGN, which includes three members in humans and two in yeast.
Sorting of lysosomal proteins.
Interaction of tyrosine-based sorting signals with clathrin-associated proteins.
The medium chains of two clathrin-associated protein complexes specifically interacted with tyrosine-based signals of several integral membrane proteins, and it is likely that the medium chains serve as signal-binding components of the clathin-dependent sorting machinery.