• Publications
  • Influence
Recognition of Polyadenylate RNA by the Poly(A)-Binding Protein
The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution and it is shown that the convex dorsum of RRM1/2 displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with translation initiation factors and regulatory proteins. Expand
Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin
It is proposed that T6SS is a multicomponent structure whose extracellular part resembles both structurally and functionally a bacteriophage tail, an efficient machine that translocates proteins and DNA across lipid membranes into cells. Expand
Protein production and purification
This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators. Expand
Mechanism of action of a flavin-containing monooxygenase.
It is proposed that FMOs exist in the cell as a complex with a reduced form of the prosthetic group and NADPH cofactor, readying them to act on substrates to eliminate nonnutritional and insoluble compounds. Expand
Periplasmic domains of Pseudomonas aeruginosa PilN and PilO form a stable heterodimeric complex.
This work shows a direct interaction between the periplasmic domains of PilN and PilO, with PilO playing a key role in the proper folding of Piln, and suggests that PilN/O heterodimers form the foundation of the inner-membrane PilM/N/ O/P complex, which is critical for the assembly of a functional T4P complex. Expand
Sequence, structure, function, immunity: structural genomics of costimulation
This review highlights unique insights that have been revealed by structures of costimulatory molecules from the immunoglobulin and tumor necrosis factor superfamilies and describes a vision for future structural and mechanistic analysis ofcostimulation. Expand
Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases.
It is demonstrated that Cas5d is a sequence-specific RNA endonuclease that cleaves CRISPR repeats and is thus responsible for processing of pre-crRNA, which generates the mature effector RNAs that target foreign nucleic acid for degradation. Expand
Crystal structure of trehalose‐6‐phosphate phosphatase–related protein: Biochemical and biological implications
We report here the crystal structure of a trehalose‐6‐phosphate phosphatase–related protein (T6PP) from Thermoplasma acidophilum, TA1209, determined by the dual‐wavelength anomalous diffraction (DAD)Expand
X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing.
It is shown that T-Rex binds to the Rex operator, and NADH but not NAD(+) inhibits T- Rex/DNA binding activity, and a mechanism for redox sensing by Rex family members is proposed by analogy with domain closure of NAD(H)-dependent enzymes. Expand
Structure of the Arginine Methyltransferase PRMT5-MEP50 Reveals a Mechanism for Substrate Specificity
Histone peptide arrays and solution assays demonstrate that PRMT5-MEP50 activity is inhibited by substrate phosphorylation and enhanced by substrate acetylation, which support a mechanism in which MEP50 binds substrate and stimulates PR MT5 activity modulated by substrate post-translational modifications. Expand