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Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR‐mediated gene disruption and other applications
TLDR
A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed and will reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications.
Functional profiling of the Saccharomyces cerevisiae genome
TLDR
It is shown that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment, and less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal Growth in four of the tested conditions.
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
TLDR
A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome), finding that 17 percent were essential for viability in rich medium.
Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence.
TLDR
A genome-wide survey of Saccharomyces cerevisiae retrotransposons offers the first opportunity to view organizational and evolutionary trends among retrotranspoons at the genome level, and it is hoped the compiled data will serve as a starting point for further investigation and for comparison to other, more complex genomes.
A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
TLDR
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection, based on the loss of orotidine-5′-phosphate decarboxylase activity, and seems applicable to a variety of eucaryotic and procaryotic cells.
An unusual form of transcriptional silencing in yeast ribosomal DNA.
TLDR
A novel form of transcriptional silencing in S. cerevisiae in the ribosomal DNA (rDNA) tandem array is identified, suggesting that a specific chromatin structure in rDNA down-regulates polymerase II promoters.
Sir2-Dependent Activation of Acetyl-CoA Synthetase by Deacetylation of Active Lysine
TLDR
It is proposed that acetylation modulates the activity of all the AMP-forming family of enzymes, including nonribosomal peptide synthetases, luciferase, and aryl- and acyl-CoA synthetasing, and imply that lysine acetylations is a common regulatory mechanism in eukaryotes and prokaryotes.
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