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The Composite Genome of the Legume Symbiont Sinorhizobium meliloti
The annotated DNA sequence of the α-proteobacteriumSinorhizobium meliloti, the symbiont of alfalfa, is presented, indicating that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution.
Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021
The presence in this replicon of a large number of nucleotide cyclases with a peculiar structure, as well as of genes homologous to virulence determinants of animal and plant pathogens, opens perspectives in the study of this bacterium both as a free-living soil microorganism and as a plant symbiont.
Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions.
Sinorhizobium meliloti is an alpha-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where
fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti.
A cascade model for fixN regulation is proposed such that fixLJ activates fixN via fixK, and fixK negatively regulates expression of the nif‐specific activator nifA as well as its own expression by autoregulation.
Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia.
It is suggested that a unique shared genetic strategy does not support symbiosis of rhizobia with legumes, and the first complete genome sequence of a beta-proteobacterial nitrogen-fixing symbiont of legume, Cupriavidus taiwanensis, reveals characteristics of a minimal rhizobium, including the most compact (35-kb) symbiotic island identified so far in any rhzobium.
FixJ: a Major Regulator of the Oxygen Limitation Response and Late Symbiotic Functions of Sinorhizobium meliloti
This study revealed that FixJ has no other direct targets besides those already known, and proposed an updated model of the FixJ regulon, involved in the regulation of functions such as denitrification or amino acid/polyamine metabolism and transport.
Transcriptome analysis of Sinorhizobium meliloti during symbiosis
The recently determined Sinorhizobium meliloti genome sequence is mined for genes related to invasion, oxidative stress protection, iron mobilization, and signaling, thus emphasizing possible common mechanisms between symbiosis and pathogenesis.