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Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.
TLDR
It is shown that some of their properties are different from tubulin, such as weak dimerization and chaperone-independent folding, however, their structure is strikingly similar to tubulin including surface loops, and BtubA/B form tubulin-like protofilaments. Expand
Novel Polyphenol Oxidase Mined from a Metagenome Expression Library of Bovine Rumen
TLDR
Kinetic, electrochemical, and EPR analyses provide unequivocal evidence that the hypothetical proteins from Bacteroides thetaiotaomicron and from E. coli, which are closely related to the deduced protein encoded by the RL5 gene, are also multicopper proteins with polyphenol oxidase activity. Expand
The Antibacterial Cell Division Inhibitor PC190723 Is an FtsZ Polymer-stabilizing Agent That Induces Filament Assembly and Condensation*
TLDR
The biochemical action of PC190723 on FtsZ parallels that of the microtubule-stabilizing agent taxol on the eukaryotic structural homologue tubulin, and it is yet to be investigated whether both ligands target structurally related assembly switches. Expand
Assembly of Archaeal Cell Division Protein FtsZ and a GTPase-inactive Mutant into Double-stranded Filaments*
TLDR
It is proposed that the primary assembly product of FtsZ is the double-stranded filament, one or several of which might form the dynamic Z ring during prokaryotic cell division. Expand
Low-resolution structures of proteins in solution retrieved from X-ray scattering with a genetic algorithm.
TLDR
Small-angle x-ray solution scattering (SAXS) is analyzed with a new method to retrieve convergent model structures that fit the scattering profiles, and the low-resolution solution structure of lysozyme has been directly modeled from its experimental SAXS profile. Expand
Tubulin homolog TubZ in a phage-encoded partition system
TLDR
Using biochemical and biophysical approaches, it is proved that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Expand
Physicochemical characterization of the heat-stable microtubule-associated protein MAP2.
TLDR
Examination of the circular dichroism of MAP2 as a function of temperature indicated that the thermal stability of this protein was probably due to its mostly unordered structure and the fact that the little ordered structure present was resistant to thermal denaturation. Expand
Magnesium-induced Linear Self-association of the FtsZ Bacterial Cell Division Protein Monomer
TLDR
It is proposed that these FtsZ oligomers and the polymers formed under assembly conditions share a similar axial interaction between monomers (like in the case of tubulin, the eukaryotic homolog of Ftsz). Expand
Interaction of epothilone analogs with the paclitaxel binding site: relationship between binding affinity, microtubule stabilization, and cytotoxicity.
TLDR
The correlation between binding, microtubule stabilization, and cytotoxicity of the compounds has been determined, and the binding constants correlate well with IC(50) values, demonstrating that affinity measurements are a useful tool for drug design. Expand
Energetics and geometry of FtsZ polymers: nucleated self-assembly of single protofilaments.
TLDR
Nucleated self-assembly of FtsZ from Escherichia coli with GTP and magnesium produces flexible filaments 4-6 nm-wide, only compatible with a single protofilament, which agrees with previous scanning transmission electron microscopy results and is supported by recent cryo-electron tomography studies of two bacterial cells. Expand
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