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Guidelines for tunneling in enzymes.
TLDR
The work identifies when nicotinamides may be vulnerable to radical mediated oxidation-reduction and merge electron-tunneling expressions with the chemical rate expressions of Eyring, and provides guidelines for the construction of new artificial oxidoreductases inspired by Nature but adopting independent design and redox engineering. Expand
Gene-Mediated Chemical Communication in Synthetic Protocell Communities.
A gene-directed chemical communication pathway between synthetic protocell signaling transmitters (lipid vesicles) and receivers (proteinosomes) was designed, built and tested using a bottom-upExpand
Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue
TLDR
It is shown that pre-treating human mesenchymal stem cells (hMSCs) with synthetic membrane-active myoglobin-polymer–surfactant complexes can provide a reservoir of oxygen capable of alleviating necrosis at the centre of hyaline cartilage. Expand
Photo-switchable phase separation and oligonucleotide trafficking in DNA coacervate micro-droplets.
TLDR
The formation and photo-switchable behaviour of light-responsive coacervate droplets prepared from mixtures of double-stranded DNA and an azobenzene cation are described, which open perspectives for the spatiotemporal control of DNA coacers and provide a step towards the dynamic regulation of synthetic protocells. Expand
Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site.
TLDR
It is concluded that site 1, but not site 2, forms part of a regulatory Ca2+-binding site, which is distinct from other previously described Ca2-binding sites. Expand
Rhodobacter sphaeroides haem protein: a novel cytochrome with nitric oxide dioxygenase activity.
TLDR
It is possible that SHP functions physiologically as a nitric oxide dioxygenase, protecting the organism against NO poisoning, and a possible mechanism for this process is proposed. Expand
Decorated Protein Networks: Functional Nanomaterials with Tunable Domain Size
TLDR
It is shown that it is possible to assemble networks of proteins of one species which the authors can decorate with another, whose coverage they can tune, and the interfaces between domains of each component can be tuned, with applications for example in energy transfer. Expand
Elementary tetrahelical protein design for diverse oxidoreductase functions.
TLDR
A man-made protein platform that reproduces many of the diverse functions of natural oxidoreductases without importing the complex and obscure interactions common to natural proteins is described. Expand
Constructing a man-made c-type cytochrome maquette in vivo: electron transfer, oxygen transport and conversion to a photoactive light harvesting maquette.
TLDR
The work described here represents a major advance in de novo protein design, offering a robust platform for new c-type heme based oxidoreductase designs and an equally important proof-of-principle that cofactor-equipped man-made proteins can be expressed in living cells, paving the way for constructing functionally useful man- made proteins in vivo. Expand
De novo protein components for oxidoreductase assembly and biological integration.
TLDR
The state of the art in de novo oxidoreductase construction is discussed, focusing on the diverse manmade componentry available and how their functions might be interfaced and integrated within living organisms. Expand
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