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Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation
TLDR
It is indicated that the molar ω2 : δ2 ratio regulates the polymerization properties of (δ•ATP•Mg2+)2 on and depolymerization from parS DNA, thereby controlling the temporal and spatial segregation of pSM19035 before cell division. Expand
Lithium blocks the PKB and GSK3 dephosphorylation induced by ceramide through protein phosphatase-2A.
TLDR
It is hypothesised that the neuroprotective action of lithium may be due to the inhibition of the PP2A activation by apoptotic stimuli. Expand
Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family
TLDR
The AsnC-asparagine structure is the first for a regulator–effector complex and is revealed as an octameric disc, and the LrpC structure reveals a stable octamer supportive of a topological role in dynamic DNA packaging. Expand
Genome engineering reveals large dispensable regions in Bacillus subtilis.
TLDR
It is shown that genome engineering is a feasible strategy for functional analysis of large gene clusters, and that removal of dispensable genomic regions may pave the way toward an optimized Bacillus cell factory. Expand
Recruitment of Bacillus subtilis RecN to DNA Double-Strand Breaks in the Absence of DNA End Processing
TLDR
It is proposed that RecN is one of the first recombination proteins detected as a discrete focus in live cells in response to DSBs and that either AddAB or RecQ(S)-RecJ are required for the generation of a duplex with a 3'-ssDNA tail needed for filament formation of RecA. Expand
Molecular analysis of the Bacillus subtilis bacteriophage SPP1 region encompassing genes 1 to 6. The products of gene 1 and gene 2 are required for pac cleavage.
TLDR
The organization of gene 1 and gene 2 of SPP1 resembles the organization of genes in the pac/cos region of different Escherichia coli double-stranded DNA phages, and it is proposed that the conserved gene organization is representative of the packaging machinery of a primordial packaging system. Expand
Visualization of DNA double‐strand break repair in live bacteria reveals dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to distinct sites on the nucleoids
TLDR
A model in which RecN that forms multimers in solution and high‐molecular‐weight complexes in cells containing DSBs initiates the formation of RCs that mediate D SB repair with the homologous sister chromosome is suggested, which presents a novel concept for DSB repair in prokaryotes. Expand
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins
TLDR
The results provide a mechanistic framework for rationalizing the coordinated events modulated by SsbA, SsbB and RecO that are crucial for RecA-dependent chromosomal transformation andRecA-independent plasmid transformation. Expand
Characterization of recombination-deficient mutants of Bacillus subtilis
TLDR
The properties of double Rec- mutants showed that recF and addA belong to different epistatic groups, whereas recF, recL, and recH fall into the same group, and more than one pathway for genetic exchange might be operative in B. subtilis. Expand
Homologous-pairing Activity of the Bacillus subtilisBacteriophage SPP1 Replication Protein G35P*
TLDR
Electron microscopic analysis shows that G35P forms a multimeric ring structure in ssDNA tails of dsDNA molecules and left-handed filaments on ssDNA, consistent with the hypothesis that the homologous pairing catalyzed by G35p is an integral part of SPP1 DNA replication. Expand
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