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Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import
It is shown that both tRNAGln species and a bacterial pre-tRNAAsp can be imported in vitro into mitochondria isolated from myoclonic epilepsy with ragged-red fiber cells if provided with sufficient ATP. Expand
The reverse transcription signature of N-1-methyladenosine in RNA-Seq is sequence dependent
A characteristic signature of m1A is found, which, in addition to an arrest rate, features misincorporation as a significant component and depends on RNA structure and on the nature of the nucleotide 3′ ofm1A in the template RNA, meaning it is sequence dependent. Expand
Saccharomyces cerevisiae imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrion.
The presence of the cytoplasmic GlnRS in the organelle and its involvement in mitochondrial Gln-tRNA synthesis is demonstrated, and a novel tRNA import mechanism is reconstituted into mitochondria by a novel mechanism that expands knowledge of RNA trafficking in the eukaryotic cell. Expand
An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA
It is shown that down-regulation of the Trypanosoma brucei tRNA-editing enzyme by RNAi leads to a reduction in both C-to-U and A- to-I editing of tRNA in vivo, providing a first line of evidence for the evolution of editing deaminases. Expand
Transfer RNA modifications: nature's combinatorial chemistry playground
Following synthesis, tRNAs are peppered by numerous chemical modifications which may differentially affect a tRNA's structure and function. Although modifications affecting the business ends of aExpand
Loss of Editing Activity during the Evolution of Mitochondrial Phenylalanyl-tRNA Synthetase*
The data indicate that the mitochondrial protein synthesis machinery lacks the tyrosine proofreading activity characteristic of cytosolic translation, suggesting that either organellar protein synthesis quality control is focused on another step or that translation in this compartment is inherently less accurate than in the cytosol. Expand
Do all modifications benefit all tRNAs?
A model in which some modifications have minor, possibly redundant, roles in specific tRNAs is suggested, consistent with the low specificity of some modification enzymes. Expand
Selective importation of RNA into isolated mitochondria from Leishmania tarentolae.
The data suggest that there is a structural requirement for importation of RNAs greater than approximately 17 nt, and that smaller RNAs are apparently nonspecifically imported. Expand
The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei*
It is shown that Isd11, in line with its strong association with Nfs, is localized in the mitochondrion of T. brucei, and raises the possibility of a connection between Fe/S metabolism and protein synthesis, helping integrate two seemingly unrelated pathways. Expand
The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria.
Efforts are underway in several laboratories to isolate and characterize specific components of the editing machinery, including an RNA ligase and an endoribonuclease, which are components of a 20S complex. Expand