The reverse transcription signature of N-1-methyladenosine in RNA-Seq is sequence dependent
- R. Hauenschild, L. Tserovski, M. Helm
- BiologyNucleic Acids Research
- 13 September 2015
A characteristic signature of m1A is found, which, in addition to an arrest rate, features misincorporation as a significant component and depends on RNA structure and on the nature of the nucleotide 3′ ofm1A in the template RNA, meaning it is sequence dependent.
Saccharomyces cerevisiae imports the cytosolic pathway for Gln‐tRNA synthesis into the mitochondrion
- B. Krett, J. Rinehart, M. A. Rubio, J. Alfonzo, D. Söll
- Biology, ChemistryGenes & Development
- 1 March 2005
Import of both cytoplasmic components for Gln‐tRNA suggests that the transamidation pathway, whose enzymes are encoded in the genome, may be conserved for an alternate function, and changes the view of the evolution of organellar protein synthesis.
Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import
- M. A. Rubio, J. Rinehart, J. Alfonzo
- BiologyProceedings of the National Academy of Sciences
- 8 July 2008
It is shown that both tRNAGln species and a bacterial pre-tRNAAsp can be imported in vitro into mitochondria isolated from myoclonic epilepsy with ragged-red fiber cells if provided with sufficient ATP.
An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA
- M. A. Rubio, I. Pastar, J. Alfonzo
- BiologyProceedings of the National Academy of Sciences
- 8 May 2007
It is shown that down-regulation of the Trypanosoma brucei tRNA-editing enzyme by RNAi leads to a reduction in both C-to-U and A- to-I editing of tRNA in vivo, providing a first line of evidence for the evolution of editing deaminases.
The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria.
- J. Alfonzo, O. Thiemann, L. Simpson
- BiologyNucleic Acids Research
- 1997
Efforts are underway in several laboratories to isolate and characterize specific components of the editing machinery, including an RNA ligase and an endoribonuclease, which are components of a 20S complex.
Transfer RNA modifications: nature's combinatorial chemistry playground
- J. Jackman, J. Alfonzo
- BiologyWiley Interdisciplinary Reviews - RNA
- 2013
Following synthesis, tRNAs are peppered by numerous chemical modifications which may differentially affect a tRNA's structure and function. Although modifications affecting the business ends of a…
Evolution of RNA editing in trypanosome mitochondria.
- L. Simpson, O. Thiemann, N. Savill, J. Alfonzo, D. Maslov
- BiologyProceedings of the National Academy of Sciences…
- 20 June 2000
Two different RNA editing systems have been described in the kinetoplast-mitochondrion of trypanosomatid protists and the possible evolutionary relationship of the C to U and U-insertion editing systems is discussed.
Do all modifications benefit all tRNAs?
- E. Phizicky, J. Alfonzo
- BiologyFEBS Letters
- 21 January 2010
Loss of Editing Activity during the Evolution of Mitochondrial Phenylalanyl-tRNA Synthetase*
- H. Roy, Jiqiang Ling, J. Alfonzo, M. Ibba
- Biology, ChemistryJournal of Biological Chemistry
- 18 November 2005
The data indicate that the mitochondrial protein synthesis machinery lacks the tyrosine proofreading activity characteristic of cytosolic translation, suggesting that either organellar protein synthesis quality control is focused on another step or that translation in this compartment is inherently less accurate than in the cytosol.
The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei*
- Zdeněk Paris, Piya Changmai, J. Lukeš
- Biology, ChemistryJournal of Biological Chemistry
- 4 May 2010
It is shown that Isd11, in line with its strong association with Nfs, is localized in the mitochondrion of T. brucei, and raises the possibility of a connection between Fe/S metabolism and protein synthesis, helping integrate two seemingly unrelated pathways.
...
...