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Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase.
The results reported here suggest that azidothymidine is nonselectively phosphorylated but that the triphosphate derivative efficiently and selectively binds to the HIV reverse transcriptase. Expand
Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine.
Acycloguanosine triphosphate inhibits herpes simplex virus DNA polymerase (DNA nucleotidyltransferase) 10-30 times more effectively than cellular (HeLa S3) DNA polymerases, contributing to the drug's selectivity. Expand
Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity.
  • P. Sherman, J. A. Fyfe
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 July 1990
The purified human immunodeficiency virus integration protein, a potential target for selective antiviral therapy, was expressed in Escherichia coli and selectively cleaved double-stranded DNA oligonucleotides that mimic the U3 and the U5 termini of linear HIV DNA. Expand
Enzymatic assay for deoxyribonucleoside triphosphates using synthetic oligonucleotides as template primers.
This modified assay for deoxyribonucleoside triphosphates has been improved by using synthetic oligonucleotides of a carefully defined sequence as template primers for DNA polymerase to obtain the lowest background reaction and highest sensitivity. Expand
Metabolic activation of the nucleoside analog 9-[( 2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine in human diploid fibroblasts infected with human cytomegalovirus.
9-[( 2-Hydroxy-1-(hydroxymethyl)ethoxy]-methyl)guanine (BW B759U) is a more potent inhibitor of human cytomegalovirus (HCMV) in vitro than is the related nucleoside analog acyclovir (ACV). BW B759UExpand
Thymidine kinase from herpes simplex virus phosphorylates the new antiviral compound, 9-(2-hydroxyethoxymethyl)guanine.
Low acyclo-Guo phosphorylating levels in cells infected with either of two herpes simplex virus mutants or with vaccinia virus correlated with low effectiveness of the compound against the virus in tissue culture. Expand
A human cytomegalovirus mutant resistant to the nucleoside analog 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) induces reduced levels of BW B759U triphosphate.
We have isolated a human cytomegalovirus mutant that is resistant to the antiviral drug 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U), yet exhibits wild-type sensitivity toExpand
Antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (BW A509U)
DNA chain terminator in the in vitro DNA polymerization reaction catalyzed by the Klenow fragment of E. coli DNA polymerase I may explain the lethal properties of this compound against susceptible microorganisms. Expand
Human immunodeficiency virus type 1 integration protein: DNA sequence requirements for cleaving and joining reactions
Using purified integration protein (IN) from human immunodeficiency virus (HIV) type 1 and oligonucleotide mimics of viral and target DNA, we have investigated the DNA sequence specificity of theExpand
The anti-hepatitis B virus activities, cytotoxicities, and anabolic profiles of the (-) and (+) enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine
The anti-hepatitis B (anti-HBV) activities of the (-) and (+) enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine (2'-deoxy-3'-thia-5-fluorocytosine [FTC]) were studied byExpand