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Cell wall modifications of bean (Phaseolus vulgaris) cell suspensions during habituation and dehabituation to dichlobenil.
Analysis of habituated cell walls by Fourier transform infrared spectroscopy and cell wall fractionation revealed a reduced amount of cellulose and hemicelluloses, mainly xyloglucan, qualitative and quantitative differences in pectin levels, and a non-crystalline and soluble beta-1,4-glucan. Expand
FTIR spectroscopy monitoring of cell wall modifications during the habituation of bean (Phaseolus vulgaris L.) callus cultures to dichlobenil
FTIR spectroscopy associated with a set of statistical tools is a powerful method for analyzing in muro–and more rapidly–the changes in polysaccharides related to dichlobenil habituation, and that it could be used in the future to identify cell wall changes related to habituation to other herbicides or stress factors. Expand
The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors
This mini-review examines the use of FTIR spectroscopy in conjunction with multivariate analyses to monitor cell wall changes related to the exposure of diverse plant materials to cellulose biosynthesis inhibitors (CBIs) and the habituation/dehabituation of plant cell cultures to this kind of herbicides. Expand
Characterization of cell walls in bean (Phaseolus vulgaris L.) callus cultures tolerant to dichlobenil.
Cell wall fractionation showed that in tolerant cell walls the xyloglucan-cellulose network of non-tolerant cell walls was partly replaced by a pectin-rich network mainly formed of cross-linked polyuronides with a large proportion of homogalacturonan. Expand
Novel type II cell wall architecture in dichlobenil-habituated maize calluses
The results prove that the architecture of type II cell walls is able to compensate for deficiencies in cellulose content with a more extensive and phenolic cross-linked network of arabinoxylans, without necessitating β-glucan or other polymer enhancement. Expand
Immunocytochemical characterization of the cell walls of bean cell suspensions during habituation and dehabituation to dichlobenil
The cell walls from bean cell suspensions with initial levels of habituation to dichlobenil had decreased levels of cellulose, but this effect lessened with increasing numbers of subcultures, and a decrease in the cell wall arabinogalactan protein (AGP) labelling was observed both in cell walls and in the culture medium. Expand
Unraveling the biochemical and molecular networks involved in maize cell habituation to the cellulose biosynthesis inhibitor dichlobenil.
A proteomic analysis revealed that habituation to DCB is linked to modifications in several metabolic pathways, and habituated cells present a reduction in glutathione S-transferase detoxifying activity and antioxidant activities. Expand
Characterization of structural cell wall polysaccharides in cattail (Typha latifolia): Evaluation as potential biofuel feedstock.
The results showed that common cattail cellulose content was high for plants in the order Poales and was accompanied by a small amount of cross-linked polysaccharides, which identifies commoncattail as a promising plant for use as potential bioethanol feedstock. Expand
High peroxidase activity and stable changes in the cell wall are related to dichlobenil tolerance.
Results reported here indicate that the habituation of bean cultured cells to dichlobenil relied partially on a stable change in the cellulose biosynthesis complex and is associated with high guaiacol peroxidase activity. Expand
Cell wall modifications in bean (Phaseolus vulgaris) callus cultures tolerant to isoxaben
Cultures of bean (Phaseolus vulgaris L.) callus were adapted for growth in a 12 μM concentration of the herbicide isoxaben; 1200-fold greater than the I 50 calculated. Tolerant calluses grew slower,Expand