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Somatic mutations within tumoral DNA can be used as highly specific biomarkers to distinguish cancer cells from their normal counterparts. These DNA biomarkers are potentially useful for the diagnosis, prognosis, treatment and follow-up of patients. In order to have the required sensitivity and specificity to detect rare tumoral DNA in stool, blood, lymph(More)
This presentation discusses the NASA data assimilation project at the Data Assimilation Office at the NASA/Goddard Space Flight Center. The goal is to produce accurate gridded datasets of atmospheric fields by assimilating a range of observations along with physically consistent model forecasts. This work produces datasets that are used by the climate(More)
Herein we describe the first application of direct linear analysis (DLA) to the mapping of a bacterial artificial chromosome (BAC), specifically the 185.1 kb-long BAC 12M9. DLA is a single molecule mapping technology, based on microfluidic elongation and interrogation of individual DNA molecules, sequence-specifically tagged with bisPNAs. A DNA map with S/N(More)
A fluorescence-based integrated optics microfluidic device is presented, capable of detecting single DNA molecules in a high throughput and reproducible manner. The device integrates microfluidics for DNA stretching with two optical elements for single molecule detection (SMD): a plano-aspheric refractive lens for fluorescence excitation (illuminator) and a(More)
This article is a theoretical basis for the software implementation o f t h e P h ysical-space Statistical Analysis System (PSAS) that is used for atmospheric data analysis at the NASA Data Assimilation OOce (DAO). The PSAS implements a statistical algorithm that combines irregularly spaced observations with a gridded forecast to produce an optimal estimate(More)
This presentation discusses the NASA data assimilation project at the Data Assimilation OOce at the NASA/Goddard Space Flight Center. The goal is to produce accurate gridded datasets of atmospheric elds by assimilating a range of observations along with physically consistent model forecasts. This work produces datasets that are used by the climate research(More)
This article is a theoretical basis for the software implementation of the Physical-space Statistical Analysis System (PSAS) that is used for atmospheric data analysis at the NASA Data Assimilation OOce (DAO). The PSAS implements a statistical algorithm that combines irregularly spaced observations with a gridded forecast to produce an optimal estimate of(More)
Targeted sequencing of specific loci of the human genome is a promising approach for maximizing the efficiency of second-generation sequencing technologies for population-based studies of genetic variation. Here we describe microdroplet PCR, which performs 1.5 million separate amplifications in parallel, as an approach for enriching targeted sequences in(More)
We describe the software implementation of the Physical-space Statistical Analysis System (PSAS) version v1.5.1 at the NASA Data Assimilation OOce (DAO). This software implements a statistical algorithm that combines irregularly spaced observations with a gridded forecast to produce an optimal estimate of the state of the atmosphere. We describe how the(More)