J. S. Hopkins

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BACKGROUND Irritable bowel syndrome is a common gastrointestinal disorder, and its prevalence and demographics have been evaluated by different methodologies with varying results. AIM To evaluate irritable bowel syndrome demographic and prevalence characteristics utilizing a web-enabled panel. METHODS From an existing 150 000-member panel, 31 829(More)
OBJECTIVE The aim of this study was to evaluate the relationships between age, health status, access to care, and breast and cervical cancer screening among multiethnic elderly and nonelderly women. METHODS A structured telephone survey of a quota sample of 1,420 New York City women from four Hispanic groups (Columbian, Dominican, Puerto Rican,(More)
In many African countries where both Government resources and donor aid for the control of tsetse-transmitted trypanosomiasis are declining, there is an increasing need to identify areas where intervention is most likely to be technically, economically, socially and environmentally sustainable. Activities then can be focused so that the maximum beneŽ ts are(More)
The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Absorbance (450 nm) results for samples were expressed as percent positivity, i.e. percentage of the median absorbance result of four replicates of the strong positive control(More)
The prefemoral efferent lymphatics of sheep infected with Trypanosoma evansi and inoculated with P. haemolytica vaccine and of those given only the vaccine, were surgically cannulated to study the effects of the infection on the total cellular output and output of blast cells from the node in response to the vaccine. T. evansi delayed and depressed the(More)
The effects of Trypanosoma evansi on efferent lymphocyte phenotypes draining from a lymph node primed with Pasteurella haemolytica vaccine were studied in sheep. The prefemoral efferent lymphatic ducts of the infected sheep along with those of two uninfected sheep were surgically cannulated. Lymph was collected and lymphocytes recovered from it analysed by(More)
The effects of Trypanosoma evansi on the cellular and humoral immune responses of sheep to Pasteurella haemolytica vaccine were studied. Peripheral blood lymphocytes (PBLs) from the sheep were analysed using single and double-colour indirect immunofluorescence staining and flow cytometry to monitor changes in circulating B and T cell subsets. Serum antibody(More)
The effects of Trypanosoma evansi on the proliferative responses of ovine peripheral blood leucocytes (PBL) were examined in in vitro cell culture systems. Sheep were vaccinated against pneumonic pasteurellosis with a monovalent Pasteurella haemolytica vaccine and then infected with T. evansi TREU 2143. From 1 week post-infection (p.i.), the PBL were(More)
Mononuclear cell subpopulations in local skin reactions (chancres) in sheep infected with metacyclic forms of Trypanosoma congolense were studied by indirect immunoperoxidase staining using a panel of monoclonal antibodies (MoAbs) specific for ovine leucocyte subsets. Morphometric analysis revealed significant increases in numbers of cells expressing CD5,(More)
Intradermal inoculation of sheep with culture-derived metacyclic forms of Trypanosoma congolense resulted in the development of localized skin reactions (chancres) and enlargement of the draining lymph nodes 7 days after infection. Changes in the expression of surface antigens of lymphocytes in lymph leaving the affected skin reactions and in the associated(More)