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The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Absorbance (450 nm) results for samples were expressed as percent positivity, i.e. percentage of the median absorbance result of four replicates of the strong positive control(More)
Mononuclear cell subpopulations in local skin reactions (chancres) in sheep infected with metacyclic forms of Trypanosoma congolense were studied by indirect immunoperoxidase staining using a panel of monoclonal antibodies (MoAbs) specific for ovine leucocyte subsets. Morphometric analysis revealed significant increases in numbers of cells expressing CD5,(More)
The prefemoral efferent lymphatics of sheep infected with Trypanosoma evansi and inoculated with P. haemolytica vaccine and of those given only the vaccine, were surgically cannulated to study the effects of the infection on the total cellular output and output of blast cells from the node in response to the vaccine. T. evansi delayed and depressed the(More)
Intradermal inoculation of sheep with culture-derived metacyclic forms of Trypanosoma congolense resulted in the development of localized skin reactions (chancres) and enlargement of the draining lymph nodes 7 days after infection. Changes in the expression of surface antigens of lymphocytes in lymph leaving the affected skin reactions and in the associated(More)
BACKGROUND Innovations in shunt technology and neuroendoscopy have been increasingly applied to shunt management. However, the relative life span of shunts and the etiology of shunt failure have not been characterized recently. METHODS We reviewed the records of all shunting procedures at our institution between January 1992 and December 1998. Independent(More)
BACKGROUND Irritable bowel syndrome is a common gastrointestinal disorder, and its prevalence and demographics have been evaluated by different methodologies with varying results. AIM To evaluate irritable bowel syndrome demographic and prevalence characteristics utilizing a web-enabled panel. METHODS From an existing 150 000-member panel, 31 829(More)
In many African countries where both Government resources and donor aid for the control of tsetse-transmitted trypanosomiasis are declining, there is an increasing need to identify areas where intervention is most likely to be technically, economically, socially and environmentally sustainable. Activities then can be focused so that the maximum bene ts are(More)
OBJECTIVE The aim of this study was to evaluate the relationships between age, health status, access to care, and breast and cervical cancer screening among multiethnic elderly and nonelderly women. METHODS A structured telephone survey of a quota sample of 1,420 New York City women from four Hispanic groups (Columbian, Dominican, Puerto Rican,(More)
BACKGROUND Endoscopic placement of ventriculoperitoneal (VP) shunt catheters in pediatric patients has been increasingly used in an attempt to minimize the unacceptably high rates of revision. Although this procedure carries an increased expense, there is currently no evidence to support an improved long-term outcome. This paper compares the rates of(More)
This paper reports on changes in the lymphocyte composition of the peripheral blood in sheep infected with Trypanosoma evansi. In addition, parasite-specific IgG1 and IgM antibody responses were monitored using a double-sandwich enzyme-linked immunosorbent assay (ELISA) technique. Eight sheep were infected with 2 x 10(6) T. evansi TREU 2143. The infection(More)