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The intranuclear distribution of nuclear matrix-associated protein p107 and the 28-kD Sm antigen of U-snRNPs have been studied using double-label immunofluorescence and immunoperoxidase electron microscopy. In interphase nuclei of HeLa cells, Novikoff hepatoma cells, and rat kangaroo kidney cells, p107 was confined to discrete interchromatin domains. The(More)
The synthesis, turnover, and expression of all the major high mobility group (HMG) chromosomal proteins was studied in different rat skeletal myogenic cell lines. Whereas pulse-chase experiments revealed a similar half-life (greater than 2 cell generations) for all the HMG proteins in both L8 myoblasts and myotubes, [3H]lysine incorporation data indicated a(More)
  • J S Bhorjee
  • 1981
The phosphorylation of the high-mobility group (HMG) proteins at different stages of the cell cycle was studied in synchronized HeLa cells. HMG proteins were extracted and analyzed by NaDodSO4/polyacrylamide gel electrophoresis. Although the molecular weight distribution of HMGs remains unchanged, their total amounts increase by as much as 20-25% in the G1(More)
Antibodies and inhibitors have been used to study the process of nuclear reactivation following the fusion of chick erythrocytes with mouse L cell cytoplasts. Immunofluorescence results showed that a monoclonal antibody against a DNA 'tight-binding' protein from HeLa chromatin as well as an anti-Sm human serum failed to bind to the unreactivated erythrocyte(More)
Chromatin was isolated from synchronized HeLa cells at different stages of the cell division cycle and fractionated into DNA, histones, and nonhistone proteins. Electrophoresis of the nonhistone proteins in sodium dodecyl sulfate-polyacrylamide gels revealed a highly reproducible pattern of 22 bands, having estimated molecular weights of 15,000-180,000,(More)
The class of nonhistone chromosomal proteins that remains bound to DNA in chromatin in the presence of 2.5 M NaCl-5 M urea has proven refractile to biochemical analysis. In order to study its role in chromatin organization, we have produced monoclonal antibodies that are specific for the HeLa DNA-protein complex that remains after extraction of chromatin(More)