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Guinea pigs, weighing 250-350 g, were infected with approximately 5,000 of live germs M- tuberculosis H 37 Rv grown 10 days in deep culture of liquid semisynthetic medium according to Sula. The infection was performed subcutaneously in inquinal region. For the therapy following phages were used: DS-6A, GR-21/T, My-327 injected twice a week subcutaneously in(More)
Numerous signs of pathological alteration were induced in the pseudostratified ciliated epithelium by a 2-hour exposure to 90% oxygen. The ciliary border injury was especially conspicuous, the mean number of cilia in a given area was significantly (p less than 0.005) reduced and morphological signs of impaired self-cleaning ability were revealed. Ciliated(More)
Serious damage to the tracheal epithelium due to bronchoalveolar lavage (BAL) was recorded. Immediately after BAL 99 +/- 2% of goblet cells were exhausted and degenerated. Their regeneration began 24 h after BAL resulting in hyperplasia of goblet cells with the formation of endoepithelial mucous glands. The most pronounced injury to the ciliated cells was(More)
Phage typing of mycobacteria is a new technique not yet widely used for classification and intraspecies differentiation. Systematic long-term studies organized by the WHO have succeeded, using a battery of 11 different mycobacterial phages, in dividing M. tuberculosis into 3 different phage sub-groups, preliminarily labelled as A, B and C. A special case of(More)
The colony microstructure of the laboratory strain Mycobacterium leprae murium "Douglas" cultivated on Ogawa's egg medium was examined. A bioptical sample from the liver of a white mouse subcutaneously infected and observed for ten months was used as inoculum. The inoculum contained 5.2 X 10(9) acidfast rods. The Ogawa's media were incubated in 5%(More)
The authors studied the course of the repair of changes induced in the rabbit tracheal epithelium by saline lavage of the airways. The tracheal epithelium was examined 2, 24, 48 and 72 hours after treatment. Saline lavage stimulated the goblet cells to instantaneous discharge of their secretion. 2 hours after treatment 98 +/- 3% of the goblet cells were(More)