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Computer visualization of three-dimensional image data using IMOD.

• Jens Kremer, David N. Mastronarde, J Richard McIntosh
• Journal of structural biology
• 1996

We have developed a computer software package, IMOD, as a tool for analyzing and viewing three-dimensional biological image data. IMOD is useful for studying and modeling data from tomographic,… (More)

The molecular architecture of axonemes revealed by cryoelectron tomography.

• Daniela Nicastro, Charles Schwartz, Jason Pierson, Richard J. Gaudette, Mary E Porter, J Richard McIntosh
• Science
• 2006

Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme. Here we describe the three-dimensional… (More)

Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle

• Mark Winey, Cindy L Mamay, +4 authors J Richard McIntosh
• The Journal of cell biology
• 1995

The three dimensional organization of microtubules in mitotic spindles of the yeast Saccharomyces cerevisiae has been determined by computer-aided reconstruction from electron micrographs of serially… (More)

High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae.

• Eileen O'Toole, Mark Winey, J Richard McIntosh
• Molecular biology of the cell
• 1999

The spindle pole body (SPB) is the major microtubule-organizing center of budding yeast and is the functional equivalent of the centrosome in higher eukaryotic cells. We used fast-frozen,… (More)

Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell

• Mark S Ladinsky, David N. Mastronarde, J Richard McIntosh, Kathryn E. Howell, L Andrew Staehelin
• The Journal of cell biology
• 1999

Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at approximately… (More)

A Cytoplasmic Dynein Heavy Chain Is Required for Oscillatory Nuclear Movement of Meiotic Prophase and Efficient Meiotic Recombination in Fission Yeast

• Ayumu Yamamoto, Robert R. West, J Richard McIntosh, Yasushi Hiraoka
• The Journal of cell biology
• 1999

Meiotic recombination requires pairing of homologous chromosomes, the mechanisms of which remain largely unknown. When pairing occurs during meiotic prophase in fission yeast, the nucleus oscillates… (More)

The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds.

• Ruxian Ding, Robert R. West, D M Morphew, Berl R Oakley, J Richard McIntosh
• Molecular biology of the cell
• 1997

The cycle of spindle pole body (SPB) duplication, differentiation, and segregation in Schizosaccharomyces pombe is different from that in some other yeasts. Like the centrosome of vertebrate cells,… (More)

Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography.

• Brad J. Marsh, David N. Mastronarde, Karolyn F. Buttle, Kathryn E. Howell, J Richard McIntosh
• Proceedings of the National Academy of Sciences…
• 2001

The positional relationships among all of the visible organelles in a densely packed region of cytoplasm from an insulin secreting, cultured mammalian cell have been analyzed in three dimensions… (More)

Interpolar spindle microtubules in PTK cells

• David N. Mastronarde, Kent L. McDonald, Ruxian Ding, J Richard McIntosh
• The Journal of cell biology
• 1993

Spindle microtubules (MTs) in PtK1 cells, fixed at stages from metaphase to telophase, have been reconstructed using serial sections, electron microscopy, and computer image processing. We have… (More)

A standardized kinesin nomenclature

• Carolyn J. Lawrence, R. Kelly Dawe, +19 authors Linda Wordeman
• The Journal of cell biology
• 2004

In recent years the kinesin superfamily has become so large that several different naming schemes have emerged, leading to confusion and miscommunication. Here, we set forth a standardized kinesin… (More)

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