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- Jens Kremer, David N. Mastronarde, J Richard McIntosh
- Journal of structural biology
- 1996
We have developed a computer software package, IMOD, as a tool for analyzing and viewing three-dimensional biological image data. IMOD is useful for studying and modeling data from tomographic,… (More)
- Mark Winey, Cindy L Mamay, +4 authors J Richard McIntosh
- The Journal of cell biology
- 1995
The three dimensional organization of microtubules in mitotic spindles of the yeast Saccharomyces cerevisiae has been determined by computer-aided reconstruction from electron micrographs of serially… (More)
Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme. Here we describe the three-dimensional… (More)
- Eileen O'Toole, Mark Winey, J Richard McIntosh
- Molecular biology of the cell
- 1999
The spindle pole body (SPB) is the major microtubule-organizing center of budding yeast and is the functional equivalent of the centrosome in higher eukaryotic cells. We used fast-frozen,… (More)
- Mark S Ladinsky, David N. Mastronarde, J Richard McIntosh, Kathryn E. Howell, L Andrew Staehelin
- The Journal of cell biology
- 1999
Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at approximately… (More)
- Edward D. Salmon, R J Leslie, William M. Saxton, M L Karow, J Richard McIntosh
- The Journal of cell biology
- 1984
The rate of exchange of tubulin that is incorporated into spindle microtubules with dimeric tubulin in the cytoplasm has been measured in sea urchin eggs by studying fluorescence redistribution after… (More)
- Robert R. West, Elena Vaisberg, Ruxian Ding, P. Nurse, J Richard McIntosh
- Molecular biology of the cell
- 1998
The "cut" mutants of Schizosaccharomyces pombe are defective in spindle formation and/or chromosome segregation, but they proceed through the cell cycle, resulting in lethality. Analysis of… (More)
- Ayumu Yamamoto, Robert R. West, J Richard McIntosh, Yasushi Hiraoka
- The Journal of cell biology
- 1999
Meiotic recombination requires pairing of homologous chromosomes, the mechanisms of which remain largely unknown. When pairing occurs during meiotic prophase in fission yeast, the nucleus oscillates… (More)
- Rena Ding, Katharine L. McDonald, J Richard McIntosh
- The Journal of cell biology
- 1993
Mitotic spindles of Schizosaccharomyces pombe have been studied by EM, using serial cross sections to reconstruct 12 spindles from cells that were ultrarapidly frozen and fixed by freeze… (More)
- Carolyn J. Lawrence, R. Kelly Dawe, +19 authors Linda Wordeman
- The Journal of cell biology
- 2004
In recent years the kinesin superfamily has become so large that several different naming schemes have emerged, leading to confusion and miscommunication. Here, we set forth a standardized kinesin… (More)