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RNA interference (RNAi) is a powerful reverse genetic tool to study gene function. The data presented here show that Agrobacterium rhizogenes-mediated RNAi is a fast and effective tool to study genes involved in root biology. The Arabidopsis gene KOJAK, involved in root hair development, was efficiently knocked down. A. rhizogenes-mediated root(More)
A new method for the isolation of root hairs from the model legume, Medicago truncatula, was developed. The procedure involves the propagation of detached roots on agar plates and the collection of root hairs by immersion in liquid nitrogen. Yields of up to 40 micro g of root hair protein were obtained from 50-100 root tips grown for 3 weeks on a single(More)
OBJECTIVE The study aims to assess the treatment of progressive multiloculated hydrocephalus. In a retrospective study, the authors reviewed their experience with different treatment modalities. METHODS We have retrospectively evaluated 93 patients with progressive multiloculated hydrocephalus operated between 1988 and 2010. They represented around 2%(More)
NADPH-diaphorase is a useful technique to reveal NO producing neurons at light microscopic level (LM). A modification of the technique using the tetrazolium salt BSPT as substrate, is useful to study the ultrastructure of NO neurons. The aim of this work was to perform a detailed analysis of NADPH-diaphorase reactive neurons in rat mesencephalon both at(More)
This work describes a computer program (POTENCOR) that applying the Fast Fourier Transform and Pearson product-moment correlation, can calculate easily, fast and accurately the absolute and relative power as well as the inter- and intrahemispheric correlation between every pair of EEG signals for narrow bands and for broad bands. POTENCOR has three main(More)
Cardiac auscultation is a traditional, yet highly sensitive and specific diagnosis technique for cardiovascular diseases. We present a Matlab framework for cardiac signals processing and analysis, which includes a new toolbox specifically designed for the main processing tasks related to heart sound analysis. Existing frameworks for acoustic cardiac signal(More)
Nowadays, 10 Gb/s networks are becoming more and more widespread. Consequently, there is an increasing need for testing equipment for those networks, to measure parameters such as throughput, delay, jitter, packet loss rate, etc. Network testing equipment can be either active or passive, depending on whether it injects traffic or simply monitors packets in(More)
Plant hemoglobins (Hbs) are found in nodules of legumes and actinorhizal plants but also in non-symbiotic organs of monocots and dicots. Non-symbiotic Hbs (nsHbs) have been classified into two phylogenetic groups. Class 1 nsHbs show an extremely high O2 affinity and are induced by hypoxia and nitric oxide (NO), whereas class 2 nsHbs have moderate O2(More)