J. R. Serrano

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We have examined permeation by Ca(2+) and Ba(2+), and block by Mg(2+), using whole-cell recordings from alpha1G T-type calcium channels stably expressed in HEK 293 cells. Without Mg(o)(2+), inward currents were comparable with Ca(2+) and Ba(2+). Surprisingly, three other results indicate that alpha1G is actually selective for Ca(2+) over Ba(2+). 1) Mg(2+)(More)
The sixth transmembrane segment (TM6) of the CFTR chloride channel has been intensively investigated. The effects of amino acid substitutions and chemical modification of engineered cysteines (cysteine scanning) on channel properties strongly suggest that TM6 is a key component of the anion-conducting pore, but previous cysteine-scanning studies of TM6 have(More)
We have examined the kinetics of whole-cell T-current in HEK 293 cells stably expressing the a 1G channel, with symmetrical Na 1 i and Na 1 o and 2 mM Ca 2 1 o . After brief strong depolarization to activate the channels (2 ms at 1 60 mV; holding potential 2 100 mV), currents relaxed exponentially at all voltages. The time constant of the relaxation was(More)
We have examined the kinetics of whole-cell T-current in HEK 293 cells stably expressing the alpha1G channel, with symmetrical Na(+)(i) and Na(+)(o) and 2 mM Ca(2+)(o). After brief strong depolarization to activate the channels (2 ms at +60 mV; holding potential -100 mV), currents relaxed exponentially at all voltages. The time constant of the relaxation(More)
The 1I T-type calcium channel inactivates almost 10-fold more slowly than the other family members ( 1G and 1H) or most native T-channels. We have examined the underlying mechanisms using whole-cell recordings from rat 1I stably expressed in HEK293 cells. We found several kinetic differences between 1G and 1I, including some properties that at first appear(More)
The alpha1I T-type calcium channel inactivates almost 10-fold more slowly than the other family members (alpha1G and alpha1H) or most native T-channels. We have examined the underlying mechanisms using whole-cell recordings from rat alpha1I stably expressed in HEK293 cells. We found several kinetic differences between alpha1G and alpha1I, including some(More)
Previous attempts to identify residues that line the pore of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel have utilized cysteine-substituted channels in conjunction with impermeant, thiol-reactive reagents like MTSET+ and MTSES-. We report here that the permeant, pseudohalide anion [Au(CN)2]- can also react with a cysteine(More)
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This study examines the implementation of a waste heat recovery system on an electric hybrid vehicle. The selected waste heat recovery method operates on organic Rankine cycle principles to target the overall fuel consumption improvement of the internal combustion engine element of a hybrid powertrain. This study examines the operational principle of hybrid(More)
The latest generation of high-pressure common rail equipment now provides diesel engines possibility to apply as many as eight separate injection pulses within the engine cycle for reducing emissions and for smoothing combustion. With these complicated injection arrangements, optimizations of operating parameters for various driving conditions are(More)