J R Delfs

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Cultures derived from rat cerebral hemispheres were sequentially stained for acetylcholinesterase activity and for either somatostatin-like immunoreactivity or cholecystokinin-like immunoreactivity. Somatostatin-like immunoreactivity was found to coexist with acetylcholinesterase activity in individual neurons of several morphological subtypes, but(More)
We previously reported that alpha-motor neurons in organotypic cultures of rat spinal cord (OTC-SC) are resistant to excitotoxicity induced through NMDA receptors. Here we describe the effects of non-NMDA glutamate receptor agonists kainic acid (KA) and quisqualic acid (QUIS) on motor neurons in OTC-SC. Large ventral horn acetylcholinesterase-positive(More)
Somatostatin was applied to rat cortical neurons grown in dispersed cell culture while electrophysiological effects were monitored with intracellular recordings. The effects of somatostatin were predominantly excitatory, but they varied and were dose dependent. Somatostatin at concentrations between 100 pM and 1 microM depolarized 30 of 87 neurons, often(More)
We studied the direct effect of platelet secretory products on rat spinal cord explants. Morphological changes in the ventral horn neurons were assessed after staining for acetylcholinesterase (AChE). Compared to control, exposure to platelet secretory products was associated with a significant decrease in the number of AChE-positive neurons per ventral(More)
Transverse sections of postnatal rat spinal cord have been cultured using the organotypic roller tube method. These explant cultures retain identifiable anatomical landmarks, allow identification of individual neurons, can be maintained for up to 8 weeks, and undergo maturational changes in vitro. Putative ventral horn motoneurons were identified in these(More)
Cholecystokinin-octapeptide (CCK-8) was applied by local microperfusion techniques to neurons cultured from fetal rat cerebral cortex while membrane properties and synaptic activity were monitored with intracellular recording techniques. There were no consistent effects of CCK-8 on resting membrane potential, membrane input resistance, or excitability of(More)
Neurotoxic effects of excitatory amino acid (EAA) receptor agonist N-methyl-D-aspartic acid (NMDA) and its antagonists on ventral horn cholinergic neurons were studied in organotypic rollertube cultures of spinal cord (OTC-SCs) using biochemical assays of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity, and AChE histochemistry.(More)
Cholecystokinin-octapeptide-like-immunoreactivity (CCK-8-LI) is demonstrable by the indirect immunofluorescent technique in cerebral cortical neurons cultured from fetal rats. Prominent and widespread staining is seen in widely ramifying cellular processes throughout the cultures. Staining in neuronal cell bodies is enhanced by treatment of the cultures(More)
Rat cortical neurons in culture have morphological, physiological and biochemical properties similar to their counterparts in situ. Neuropeptides are synthesized by these cultures and can in situ. Neuropeptides are synthesized by these cultures and can be localized to individual neurons. One such peptide, somatostatin, is produced in relatively large(More)
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