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Both sequence-based and activity-based exploitation of environmental DNA have provided unprecedented access to the genomic content of cultivated and uncultivated microorganisms. Although researchers deposit microbial strains in culture collections and DNA sequences in databases, activity-based metagenomic studies typically only publish sequences from the(More)
Soil microbial diversity represents the largest global reservoir of novel microorganisms and enzymes. In this study, we coupled functional metagenomics and DNA stable-isotope probing (DNA-SIP) using multiple plant-derived carbon substrates and diverse soils to characterize active soil bacterial communities and their glycoside hydrolase genes, which have(More)
In the federally approved Comprehensive Everglades Restoration Plan, CERP, restoration of the South Florida ecosystem is a major task for the U.S. Army Corps of Engineers and the South Florida Water Management District. To achieve this, it is desired to develop a regional-scale watershed model that is first-principle, physics-based, and capable of(More)
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