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OBJECTIVES To determine the pediatric reference ranges for iron, cortisol, CK, CKMB, and troponin I. METHODS Iron and CK were measured on the Vitros analyzer (Johnson and Johnson) while CKMB, troponin I, and cortisol were measured on the Immuno I (Bayer Corp.). Pediatric reference ranges were determined on hospitalized patients using the Hoffmann(More)
OBJECTIVES To determine the effect of gestational age and birth weight (BW) on troponin I (TnI) and creatine kinase MB fraction (CKMB) levels during the first year of life. METHODS Troponin I and CKMB levels were determined in infants less than 1 year of age using the Immuno I (Bayer Corp.). RESULTS Troponin I fractions were greatest in the preterm(More)
The 5-hydroxytryptamine1C (5-HT1C) receptor shares many features with the 5-HT2 receptor. To determine if the regulation of the sites is also similar we studied the effects of chronic treatment with drugs active at 5-HT1C/2 receptors on [3H]mesulergine-labelled 5-HT1C binding sites in spinal cord. The 5-HT receptor agonists 1-(3-chlorophenyl)piperazine(More)
OBJECTIVE To evaluate the Point-of-Care (POC) i-STAT system for measuring blood gases (pH, pCO2, pO2) and whole blood electrolytes (sodium, Na+, potassium, K+ and ionized calcium, iCa2+) in the neonatal and pediatric intensive care units. DESIGN AND METHODS The i-STAT system was evaluated for imprecision, necessity of running quality control and accuracy.(More)
We describe a quantitative radioreceptor assay (RRA) for quantifying FK-506 in whole blood. FK-506 extracted from whole blood with a cyclohexyl-sorbent column competes with [3H]dihydro-FK-506 for binding to a partially purified preparation of FK-506 binding protein (FK-BP). Free and protein-bound FK-506 are separated on LH 20 Sephadex chromatographic(More)
A recent discovery of aromatase crystal structure triggered the efforts to design novel aromatase inhibitors for breast cancer therapy. While correlating docking scores with inhibitory potencies of known ligands, feeble robustness of scoring functions toward prediction was observed. This prompted us to develop new prediction models using stepwise regression(More)
OBJECTIVES There is a controversy regarding the role of cyclosporine (CsA) metabolites in both immunosuppression and toxicity, and measurement of the parent drug is commonly recommended. High performance liquid chromatography (HPLC) is the method commonly used for specific measurement of the parent drug, but is very time consuming. Antibody techniques are(More)
OBJECTIVES Tacrolimus (FK506) is an immunosuppressive drug with great clinical promise. There is a controversy regarding the role of tacrolimus metabolites in immunosuppression and toxicity, and immunoassays and immunophilin binding assays have not been adequately tested for metabolite cross-reactivity. Methods are limited to HPLC and HPLC-MS for(More)
To study the regulation of 5-HT1A receptors in the brainstem, the region most relevant to the serotonin syndrome and to serotonin-responsive human myoclonic disorders, we chronically treated rats with various 5-HT1A agonists and labeled 5-HT1A sites with [3H]8-OH-DPAT. Daily injection for 30 consecutive days of 10 mg/kg ip 8-OH-DPAT (pre- and post-synaptic(More)