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Recent growth studies in children suggest that there is no threshold for adverse effects from the universal exposure to inorganic lead. The biochemical mechanisms mediating low-level toxicity are unclear, but in several biological systems, lead alters calcium-mediated cellular processes and may mimic calcium in binding to regulatory proteins. Here we(More)
We investigated the effects of inorganic lead upon calcium-, phospholipid-dependent protein kinase (protein kinase C) in brain microvessels isolated from 6-day-old rat pups. We found that (a) in broken cell preparations, lead at micromolar concentrations activates this enzyme to an extent equivalent to that of micromolar calcium (10.3 +/- 1.3 and 9.2 +/-(More)
representing a major shift for the official Journal of the International College of Neuropsychopharmacology (CINP). Moving the journal from a traditional subscription-based publication model to an (OA) model means anyone in the world with Internet access can read and make use of all the important findings presented in the journal, free of charge. This new(More)
Alterations of both cyclic AMP and calcium metabolism have been found in many cell types from patients with cystic fibrosis (CF). Several groups have reported an increase in intracellular cyclic AMP in CF fibroblasts after stimulation with isoproterenol (1,2). These results have been confirmed by Markovac et al. and are reported in the accompanying paper(More)
We investigated the activation of protein kinase C in microvessels isolated from rat brain. We found that unstimulated kinase activity in microvessels from immature animals is soluble while that from adults is particulate. The tumor promoter, phorbol 12-myristate 13-acetate, and the diacylglycerol analog, 1-oleoyl-2-acetyl-sn-glycerol, caused the(More)
The possibility of altered B-adrenergic receptors and/or adenyl cyclase activity in fibroblasts from patients with cystic fibrosis (CF) has been raised by several groups of investigators. Buchwald (1) reported a greater increase in CAMP’ after isoproterenol stimulation in CF than in normal fibroblasts and this was confirmed by Roscher et al. (2) for CF and(More)
The effects of the mouse major histocompatibility complex, H-2, on phospholipid methyltransferase I and II activities were investigated on hepatocyte membranes from inbred, congenic and recombinant strains. Each methyltransferase was assayed individually by measuring the incorporation of radiolabel from S-adenosyl-L-[methyl-3H]methionine into endogenous(More)
Transforming growth factor beta (TGF-beta) is a regulatory peptide found in many normal and neoplastic tissues, including brain, with a diverse range of cellular effects. The transmembrane biochemical signals by which TGF-beta exerts these effects and the second messenger systems that may amplify them are unknown. We investigated the effects of TGF-beta(More)