The matrix protein VP40 coordinates numerous functions in the viral life cycle of the Ebola virus. These range from the regulation of viral transcription to morphogenesis, packaging and budding of mature virions. Similar to the matrix proteins of other nonsegmented, negative-strand RNA viruses, VP40 proceeds through intermediate states of assembly (e.g.… (More)
Rho kinase mediates the effects of inflammatory permeability factors by increasing actomyosin-generated traction forces on endothelial adherens junctions, resulting in disassembly of intercellular junctions and increased vascular leakage. In vitro, this is accompanied by the Rho kinase-driven formation of prominent radial F-actin fibers, but the in vivo… (More)
Endothelial cells (ECs) form a tight barrier to prevent vascular leakage. Although several studies reported that vascular leakage is increased during hypoxic conditions, other studies describe a protective effect. This raises the question how hypoxia specifically regulates adherens junction integrity and thus endothelial barrier function. Here, we… (More)
[This corrects the article DOI: 10.1371/journal.pone.0157233.].
BACKGROUND Microvascular injury (MVI) after coronary ischemia-reperfusion is associated with high morbidity and mortality. Both ischemia and reperfusion are involved in MVI, but to what degree these phases contribute is unknown. Understanding the etiology is essential for the development of new potential therapies. METHODS AND FINDINGS Rats were divided… (More)
Endothelial barrier disruption and vascular leakage importantly contribute to organ dysfunction during inflammation in conditions like sepsis and acute respiratory distress syndrome. We recently identified the kinase Abl-related gene (Arg/ABL2) as mediator of endothelial barrier disruption, but its relevance in vivo and the mechanisms by which Arg mediates… (More)
The stimulation of granulocyte macrophage-colony stimulating factor (GM-CSF) by interleukin-1 (IL-1) has been shown to be counteracted in different mesenchymal cell systems by cyclic adenosine monophosphate (cAMP) agonists. The aim of this study was the evaluation of different cAMP agonists on GM-CSF expression in human bone marrow stromal cells. Incubation… (More)