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Isocratic high-performance liquid chromatography methods are described for separating the major classes of phospholipids and for isolating the individual molecular species of phospholipids. Fractionation of a total lipid extract of rat liver on a silica column resulted in quantitative recoveries of all major phospholipids with preservation of their fatty(More)
BACKGROUND/AIMS Polyunsaturated soybean lecithin (55%-60% phosphatidylcholine [PC]) protects against fibrosis in alcohol-fed baboons. The present study was undertaken to determine whether PC is the active agent. METHODS Virtually pure PC (equivalent to that contained in the lecithin) was administered for up to 6.5 years with or without alcohol, and the(More)
In response to a diet enriched in saturated fat and cholesterol (CH), male Syrian hamsters develop hyperlipemia and changes of early atherosclerosis. However, it has not been determined if female hamsters are equally susceptible to an atherogenic diet. Male and female hamsters of the F1B hybrid strain (Bio Breeders, Fitchburg, MA) were fed either a chow(More)
Although studies have shown that saturated and polyunsaturated fats can mediate plasma lipid and apolipoprotein (apo) concentrations at the mRNA level, there is little data on the role of monounsaturated fats. We determined hepatic lipid and apo mRNA levels in 10 cynomolgus monkeys fed three diets that provided 30% of energy as fat with 0.1% cholesterol by(More)
Studies were performed in rats to quantitate the changes in the concentration of serum triglycerides (TGs) of endogenous and exogenous origin after the acute intravenous injection of TG-rich emulsion particles. Emulsions were prepared to approximate chylomicrons and to contain a TG with a single fatty acid that could be traced during its clearance from the(More)
Biliary cholesterol secretion is ordinarily tightly coupled to phosphatidylcholine (PC) secretion. Bile PCs are distinct in composition and predominantly composed of molecular species with 16:0 in the sn-1 position and 18:2 and 18:1 in the sn-2 position. In an attempt to acutely change the composition of biliary PCs and to assess the effect of a change in(More)
Unesterified cholesterol (UC) that is taken up by the liver from lipoproteins is rapidly mixed by exchange with liver UC. Thus, it is not possible to quantitate the transport of UC from different lipoproteins into bile using radiolabeled UC. However, plant sterols do not exchange with UC and are secreted in bile with the same kinetics as UC. To compare the(More)
Marked kinetic differences were observed when hepatic newly synthesized cholesterol and preformed cholesterol were separately radiolabeled and separately traced into bile. Whereas newly synthesized cholesterol was not evenly distributed throughout the liver but was preferentially secreted into bile, preformed cholesterol was in near-complete equilibrium in(More)
Bile salts are necessary for the secretion of phosphatidylcholines (PCs) in bile and result in the selective secretion of highly hydrophilic molecular species of PC that contain a 16:0 acyl group. To determine the effect of bile salt on the secretion of PCs in lipoproteins, isolated rat livers were perfused with and without taurocholate. The PC composition(More)