J M Amabis

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Injection of ecdysterone into mid-fourth instar larvae of Trichosia pubescens brought about drastic changes in the puffing pattern of the chromosomes of the salivary gland. The puff sequence induced by the hormone is quite similar to that which normally occurs at the end of larval life. Three classes of puffs were observed: the early induced, the late(More)
Trichosia pubescens larvae were ligated at different prepupal developmental stages in order to investigate whether ecdysterone acts just as a trigger or whether its presence in the hemolymph is constantly necessary for the induction of the late puffs, in particular the DNA puffs. The larvae were ligated in the middle of the body being sure that the salivary(More)
An antiserum (called AScE/D) against the semiconserved D-domain of a Chironomus tentans ecdysone receptor protein (cEcR) gave indirect immunofluorescence signals at DNA puff sites in Trichosia pubescens. The signals varied in maximum intensity at different DNA puff sites. Control experiments using the secondary rhodamine-labeled anti-rabbit IgG alone,(More)
By using the techniques of ligation of the larvae (brain and endocrine glands extirpation) and salivary gland implantation, the hormonal dependence of the activity of certain puffs of Rhynchosciara was investigated. Our results have shown that the puffing behaviour--activation and deactivation--varies according to the developmental stage in which the larvae(More)
During the course of experiments with larvae of Trichosia pubescens, we have unexpectedly found that diethyl ether or chloroform anesthesia induces a large puff in a specific band in the polytene chromosomes of the salivary glands. This puff develops a few minutes after the treatment, attaining its maximum size after 60-100 min, and regresses completely 200(More)
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