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  • Vladimír Vilím, J Krajícková
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1991 (First Published: 1 August 1991)
  • A procedure is described for the preparation of 1.32-10% polyacrylamide gradient gels. Loose polyacrylamide gel on the top side of the gradient was stabilized with a layer of 0.4% agarose gel whichContinue Reading
  • Vladimír Vilím, J Krajícková
  • Medicine, Chemistry
  • The Biochemical journal
  • 1991 (First Published: 1 February 1991)
  • Two specimens of human articulage were successively extracted with solutions of phosphate-buffered saline (PBS), 7 M-urea and 4 M-guanidine hydrochloride (Gdn-HCl). Proteoglycans from individualContinue Reading