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Peroxisomes catalyze the beta-oxidation of fatty acids but their quantitative role in fatty acid catabolism in the intact hepatocyte is not yet clarified. In the present study peroxisomal beta-oxidation of [1-14C]palmitate was quantitated in hepatocytes without the use of metabolic inhibitors. It was assumed that acetyl-CoA formed by peroxisomal(More)
Isolated hepatocytes from female rats were cultured in HI-WO/BA medium for 6 days. To the medium was added oleate, ethanol, dexamethasone and insulin. With oleate To alone, triacylglycerol accumulated; ethanol augmented the accumulation by 90%. To the best of our knowledge, this is the first demonstration that ethanol in vitro increases the content of(More)
The application of radiolabeled fatty acids to measurements of fatty acid oxidation is discussed and a method for measuring the rate of beta-oxidation and of acetyl-CoA oxidation to CO2 is described. In hepatocytes from starved or fed rats, ethanol inhibited total beta-oxidation in the presence of 1.3 mM palmitate by 22% and 25%, respectively. If changes in(More)
Calculations based on net changes and [1-14C]oleate incorporation data suggest that fatty acids are the only precursor of acetylCoA and that extensive lipolysis takes place in hepatocyte cultures incubated with 1 mM oleate in the absence or presence of ethanol. The effect of 4-methylpyrazole on fatty acid metabolism as affected by ethanol has been studied.(More)
Isolated rat hepatocytes were cultured in a modified HI-WO/BA medium for 16 h. In the following 24 h oleate or oleate plus ethanol was added to the medium. After this period the medium was changed again and the cultures were further incubated with [1-14C]oleate alone or with [1-14C]oleate plus ethanol for 6 h. This allowed a comparison of effects of(More)
Reactive free oxygen radicals are formed in the reactions involved in normal cell metabolism. This formation is closely regulated e.g. by dietary antioxidants. Present knowledge suggests that an imbalance, with surplus of free radicals, can play a role in the pathogenesis of certain types of cancer, atherosclerosis, and cataract. A number of epidemiological(More)
1. CoA, acetyl-CoA, long-chain acyl-CoA, carnitine, acetylcarnitine and long-chain acylcarnitine were measured in rat liver under various conditions. 2. Starvation caused an increase in the contents of these intermediates, except that of carnitine. 3. A single dose of ethanol had no effect on CoA content, whereas those of acetyl-CoA, acetylcarnitine and(More)
1. In the preceding paper [Kondrup (1979) Biochem. J.184, 63-71] the separation of two major fractions of hepatic triacylglycerol was described. One fraction contained triacylglycerol from the endoplasmic reticulum and from the Golgi apparatus. The other fraction contained triacylglycerol from the cytoplasmic lipid droplets. In the present paper possible(More)
In a previous report it was shown that ethanol increases the rate of accumulation of triacylglycerol by 90% in hepatocytes in primary culture. This represents the first known suitable model for in vitro studies of the ethanol-induced fatty liver. The biochemical alterations causing this accumulation of triacylglycerol remain to be elucidated, however. In(More)
1. The effect of ethanol on the metabolism of [1-(14)C]palmitate in rat liver was investigated in a single-pass perfusion system at concentrations of 10mm- or 80mm-ethanol and 0.2mm- or 1mm-palmitate. 2. After the perfusion the hepatic lipid was isolated in subcellular fractions. The two major fractions contained triacylglycerol from cytoplasmic lipid(More)