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There is an increased synthesis of proteins in the molecular weight region of 100,000 72,000-74,000 and 37,000 two hours after treatment of HeLa cells for 10 min at 45 degrees C. In vitro translation, using a rabbit reticulocyte cell-free protein synthesising system, of HeLa cell cytoplasmic RNA shows that the prominent 72,000-74,000 Mr heat shock protein(More)
While incubation of HeLa cells for 5--10 min at 45 degrees C does not affect subsequent cellular growth and DNA synthesis at 37 degrees C, there is an increased synthesis of specific proteins in the molecular weight region of 100 000, 72 000--74 000 and 37 000. The synthesis of these proteins reaches a maximum 2 h after the heat shock and while the protein(More)
Cloned cDNA sequences coding for specific 72 000-74 000 Mr HeLa cell heat shock proteins (HSPs) have been used as probes to examine cytoplasmic and nuclear RNA of heat shocked HeLa cells. The mRNAs for these HSPs vary widely in size. Whilst the beta-, delta- and epsilon-HSPs are encoded in mRNAs of 1.9 kb, the gamma-HSP arises from an mRNA three times that(More)
The method of differential in situ colony hybridisation and the technique of two-dimensional gel electrophoresis of polypeptides synthesized in vitro by mRNAs that hybridise specifically to recombinant plasmids bound to filter discs, have been used to isolate two cDNA HeLa heat-shock clones. Each of these two clones contains the plasmid pBR322 with a cDNA(More)
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