J. K. Disbrow

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The long-sleep (LS) and short-sleep (SS) mice represent established lines, genetically selected from a heterogeneous stock (HS) of mice for differences in the duration of the loss of righting reflex (sleep-time response) to acute ethanol administration. Presently, the dose of ethanol (20% v/v i.p.) required for approximately a 50-min sleep-time response is(More)
The 30 mM K+-induced release of endogenous glutamate has been examined in slices of cortex, hippocampus, striatum and cerebellum from "short sleep" and "long sleep" mice, lines genetically selected for differential response to acute ethanol administration. In cortex and striatum, glutamate release was significantly higher in "short sleep" mice, while in(More)
The ATP-stimulated accumulation of L-[3H]glutamate by whole brain synaptic vesicle preparations from long-sleep and short-sleep mice, lines selectively bred for difference in sleep time response to acute ethanol administration, was examined. L-[3H]Glutamate accumulation in vesicles from short-sleep mice was approximately twice that observed in vesicles from(More)
The accumulation of3H-dopamine by synaptic vesicles from rat striatum was significantly stabilized in a membrane impermeant medium. The characteristics of dopamine accumulation by striatal vesicles were quite similar to those reported for dopamine accumulation by a whole brain vesicle preparation in the same medium, and were siginificantly different from(More)
The biochemical lifetime of noradrenergic storage vesicles from rat brain is greatly extended in a medium based upon the membrane impermeable anion D-tartrate. Examination of the MgATP-dependent accumulation of (−)-3H-norepinephrine suggests that this medium may more closely approximate the intracellular environment of the storage vesicle, and may better(More)
3H-Dopamine (DA) accumulation by storage vesicles from whole rat brain was significantly stablized in a buffer system based upon the membrane-impermeant D-potassium tartrate. 3H-DA uptake saturated by twenty minutes (Km 2.1 X 10(-5)M) and remained stable for periods of 40-60 minutes. Accumulated DA was rapidly exchangeable with exogenous DA. Total levels of(More)
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