J. J. LaGuardia

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Trigeminal ganglia removed at autopsy from immunocompetent individuals without cutaneous signs of herpesvirus infection were fixed, cut into 5-μm sections, and screened at 100-μm intervals (20 adjacent sections) by PCR for latent herpes simplex type 1(HSV-1) and varicella zoster virus (VZV) DNA. Sections that contained >5 neurons with nuclei stained by(More)
Polymerase chain reaction (PCR) revealed herpes simplex virus (HSV) and varicella zoster virus (VZV) DNA in human nodose and celiac ganglia. This is the first detection of VZV DNA in ganglia of the human autonomic nervous system. The ability of reactivated VZV to produce serious, sometimes fatal neurological disease in the absence of rash, raises the(More)
Previous analyses using in situ hybridization alone or together with PCR have yielded conflicting results regarding the cell type in which latent varicella-zoster virus (VZV) resides. We separated human trigeminal ganglia (TG) into neuronal and nonneuronal fractions, followed by primary and nested PCR to quantitate VZV DNA at the single cell level. Both TG(More)
Varicella-zoster virus (VZV) causes chickenpox (varicella), becomes latent in cranial nerve and dorsal root ganglia, and can reactivate many years later to produce shingles (zoster) and postherpetic neuralgia (PHN). Elderly and immunocompromised individuals are also at risk for complications of VZV reactivation involving the central nervous system (CNS),(More)
We analyzed both trigeminal ganglia of eight post-mortem human subjects for their content of neurons and non-neuronal cells using dissociated cell suspension techniques. Neuronal counts in each of the 16 ganglia ranged from 20,000 to 35,400, with an average of 27,400 +/- 4800, and the estimated ratio of non-neuronal to neuronal cells was 100 to 1. Our(More)
The computer program HYLAS generates from a standard DNA letter sequence a three-dimensional space curve (H curve) which embodies the entire information content of the original nucleotide sequence. The program can display H curves either as two-dimensional (front and side view) projections or as stereo-pair images. The curves can be marked at specific(More)
PURPOSE Cellular mechanisms activated during seizures may exacerbate epilepsy. gamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in brain, and we hypothesized that brief epileptic seizures may reduce GABA function. METHODS We used audiogenic seizures (AGSs) in genetically epilepsy-prone rats (GEPRs) to investigate effects of seizures(More)
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