J J Devenny

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The extent of in vitro nonenzymatic glycosylation of purified rat brain tubulin was dependent on time and glucose concentration. Tubulin glycosylation profoundly inhibited GTP-dependent tubulin polymerization. Electron microscopy and NaDodSO4/polyacrylamide gel electrophoresis showed that glycosylated tubulin forms high molecular weight amorphous aggregates(More)
Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of albumin and glycosylated albumin(More)
Recent evidence supports the involvement of integrins in angiogenesis: blockade of alpha v beta 3 and alpha v beta 5 integrins disrupts angiogenesis leading to decreased blood vessel formation and hence decreased tumor growth. We hypothesized that av antagonists could inhibit tumor growth in tumor cells devoid of alpha v beta 3 integrins. We evaluated SM256(More)
Tumor growth is dependent on the balance between cell proliferation and cell death, and these events occur heterogenously within an individual tumor. We present a methodology that provides integrative information about cell kinetics, cell death, and cell growth within individual tumors in animals treated with cytotoxic chemotherapeutic agents. Using HCT-116(More)
Vesicular transport of solutes across capillary walls may be regulated by specific solute-endothelial interactions. Little data is available on the vesicular transport of serum proteins which may transit the capillary wall in situ. Capillaries were isolated from epididymal fat and incubated in fluorescent-labeled transferrin and radiolabeled sucrose.(More)
The ability of capillary endothelial vesicles to transport immunoglobulin G (IgG) across the capillary wall was investigated. Isolated capillary endothelial cells were found to endocytose fluorescent-labeled IgG via a bulk fluid-phase mechanism. When placed in marker-free medium the cells released all previously ingested label. Cationization of IgG causes(More)
Numerous studies investigating the cGMP-gated cation conductance in rod disk membranes have purported to measure efflux of Ca2+ entrapped in rod disk membrane vesicles. We have utilized sonication and osmotic shock as additional tests for sensitivity of cGMP- and A23187-induced Ca2+ release to elimination of the transvesicular Ca2+ gradient. We find that 1)(More)
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