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In the last decade, significant progress has been made in the characterization of pH regulation in nervous tissue in vitro. However, little work has been directed at understanding how pH regulatory mechanisms function in vivo. We are interested in how ischemic acidosis can effect pH regulation and modulate the extent of post-ischemic brain damage. We used(More)
We have studied the metabolic and functional effects of two new platelet-activating factor (PAF) antagonists (BN 50726 and BN 50739) and their diluent (dimethyl sulfoxide; DMSO) during reoxygenation of the 14-min ischemic isolated brain. Blood gases, EEG, auditory evoked potentials, cerebral metabolic rate for glucose (CMRglc), and cerebral metabolic rate(More)
I'm on my fourth cup of coffee this morning and feeling a bit plagiaristic, so please indulge my rip-off of Stephan Covey's popular (and terrific) book The Seven Habits of Highly-Effective People. Here are my top picks for successful C++ habits: 1. Don't expect miracles: Object-oriented programming is valuable and C++ is a good way to make a transition from(More)
A unique method for simultaneously measuring interstitial (pHe) as well as intracellular (pHi) pH in the brains of lightly anesthetized rats is described. A 4-mm microdialysis probe was inserted acutely into the right frontal lobe in the center of the area sampled by a surface coil tuned for the collection of 31P-NMR spectra. 2-Deoxyglucose 6-phosphate(More)
Nitrous oxide has been reported to act both as a stimulant and as a depressant of cerebral oxygen metabolism (CMRO2) and blood flow under a variety of experimental conditions in the intact animal. The isolated brain preparation is advantageous because it permits direct measurement of blood flow and allows the study of drug effects without interference from(More)
Sixty-four isolated canine brain preparations were subjected to either 15 or 30 min of perfusion with blood equilibrated at either Pao2 30 mmHg or Pao2 40 mmHg followed by up to 60 min of reoxygenation with blood having a Pao2 greater than 100 mmHg. Pao2 30 mmHg perfusion decreased oxygen availability and the cerebral metabolic rate for oxygen (CMRo2) to 44(More)
We have studied the effect of increased blood O2 affinity on O2 delivery to the isolated canine brain. After surgical isolation, the brain, enclosed in the calvarium, was perfused alternately from two pump-oxygenators with normal blood (P 50 [7.4] = 30 +/- 2 torr [S.D.]) and with blood whose P50 was reduced to 18 +/- 2 torr by carbamylation. [Hb], acid-base(More)
The Pulsinelli-Brierley four-vessel occlusion model was used to study the consequences of hyperglycemic ischemia and reperfusion. Rats were subjected to either 30 min of normo- or hyperglycemic ischemia or 30 min of normo- or hyperglycemic ischemia followed by 60 min of reperfusion. In some animals, 2 mg/kg BN 50739, a platelet-activating factor receptor(More)