J E de Peyer

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Modulation of ion channels is of increasing interest as it is an important step in the regulation of cellular functions. We have analysed the effect of 8-bromocyclic AMP on Ca2+ channels in cultured cardiac cells by the patch-clamp method and report here that there was a large increase in the probability of opening of the channels. On the basis of a(More)
Primary cardiac cell cultures were prepared from the hearts of neonatal rats. The patch-clamp method (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) was applied for studying whole-cell Na+ currents and single-channel Na+ currents, respectively. Whole-cell recordings yielded voltage- and time-dependent Na+ currents which could be blocked by tetrodotoxin.(More)
In strip preparations from the rabbit main pulmonary artery, it was not possible to differentiate convincingly between alpha 1- and alpha 2-adrenoceptors, when selective agonists such as methoxamine, St 587, B-HT 920, and clonidine, as well as selective antagonists such as prazosin and yohimbine were used for receptor characterization. Measurements of the(More)
  • J E de Peyer, J W Deitmer
  • The Journal of experimental biology
  • 1980
We have investigated the ability of various divalent cations to carry current through membrane channels in ciliates activated by mechanical stimulation and by membrane depolarization. Both types of channels are identified as Ca2+ channels. Whereas Ba2+ ions and Sr2+ ions can replace Ca2+ ions as charge carriers during either kind of current, Mg2+ ions can(More)
Strips of rabbit main pulmonary artery (RMPA) were used to study the effects of several agonists on tension development and membrane potential of the vascular smooth muscle cells. The following alpha-adrenoceptor agonists were employed: methoxamine and St 587 (alpha 1-selective), B-HT920 (alpha 2-selective) and clonidine, which stimulates preferentially(More)
Depolarizing voltage steps induce inward and outward currents in voltage-clamped, internally perfused neurons from the snail Helix roseneri. Addition of the catalytic subunit of cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) to the internal perfusing medium results in an increase in the net outward current, with no(More)
The resting membrane potential of the smooth muscle cells of strips of rabbit aorta varied between -50 to -60 mV. No spontaneous spike discharges were observed. The membrane of the myocytes showed a marked outward rectifying property. The rabbit aorta had cable properties with a space constant (lambda) of 1.54 +/- 0.04 mm. Parallel with a progressive(More)