Learn More
The onset of puberty is a concerted action of many factors which leads to cyclic LHRH release in rats. It has been demonstrated that; in common with vasoactive intestinal polypeptide (VIP), pituitary adenylate cyclase activating polypeptide (PACAP) is also involved in the differentiation of the central nervous system. In our previous work, it was shown that(More)
The release of growth hormone (GH) and cAMP was studied in superfused rat pituitary cells by infusing growth hormone-releasing hormone (GHRH) at different doses or a combination of GHRH and somatostatin 14 (SS-14). Three-minute pulses of GHRH caused a dose-dependent GH and cAMP release (effective concentration of 50% of the maximal biological effect is 0.21(More)
The LHRH-synthesizing neuron system was studied in young proestrous and old female rats, and in aged ovariectomized or reserpine-treated females. The medial preoptic area and septal region of old animals contains more LHRH positive perikarya compared to that of young proestrous rats. Reserpine treatment moderately increases the number of immunostainable(More)
Abstract Several details regarding the control of melatonin (MT) secretion from the pinealocytes are still to be clarified. To obtain more data on the mechanism and kinetics of MT secretion and on the interactions between bioactive materials affecting MT production, a perifusion system has been developed in our laboratory. In this dynamic in vitro system(More)
The onset of the synthesis of the releasing and inhibiting hormones, associated with their identified primary structures (LHRH, somatostatin), has been studied successfully in some vertebrates. It is known from radioimmunoassay and immunohistological studies that the synthesis of LHRH, TRH and somatostatin begins in the brain of different mammalian species(More)
The onset of hormone synthesis in LH-RH and somatostatin neurons of the brain was studied in rats using the unlabelled antibody-PAP immunohistochemical method. LH-RH appeared in detectable amounts first in neuronal perikarya in the preoptic region of fetuses sacrificed on the 17th day of gestation. Minute amounts of LH-RH could already be detected in some(More)
  • 1