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The distribution of monoamines in the brain of Triturus alpestris tadpoles was investigated with the Falck-Hillarp histofluorescence method. This study concerns especially the diencephalon where is observed the major part of the monoaminergic (MA) neurons. MA fiber tracts are found in the striatum, the septal nucleus and the medial and lateral forebrain(More)
In the brain of Rana temporaria, two distinct systems reactive with alpha- and beta-endorphin antisera, respectively, and with a met-enkephalin antiserum, have been detected immunohistochemically. Neurons reacting with alpha- and beta- endorphin antisera are located (1) in the preoptic nucleus, and (2) in the pars ventralis of the tuber cinereum.(More)
For simultaneous ultrastructural localization of intracellular peptides, protein A-gold techniques or immuno-gold techniques have generally been applied. The present study reports a double immunostaining procedure for simultaneous visualization of two hypophysial hormones (prolactin and corticotropin) on a single ultrathin section of the pars distalis of an(More)
The triple ultrastructural immunocytochemical labeling technique described here is based on the use of three different antisera raised in two different animal species: rabbit anti-corticotropin (R-ACTH), guinea pig anti-prolactin (GP-LTH) and rabbit anti-gonadotropin (R-LH beta). Staining is carried out on both sides (A and B) of the same tissue section.(More)